cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas

Naoki Itoh, Keisuke G. Takahashi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A novel lysozyme cDNA from the Pacific oyster, Crassostrea gigas, was identified. This second lysozyme from the Pacific oyster was designated as CGL-2. The complete CGL-2 cDNA sequence comprises of 536 bp, and 429 bp of the open reading frame encodes 147 bp of amino acid residues. Estimated CGL-2 molecular characteristics (isoelectric point and numbers of peptide recognition sites) resembled those of cv-lysozyme 2, a digestive lysozyme of the eastern oyster, Crassostrea virginica. Moreover, CGL-2 is phylogenetically homologous to the cv-lysozyme 2, indicating that CGL-2 and cv-lysozyme 2 evolved from the same ancestor protein for adaptation to the digestive environment. In situ hybridization revealed that the CGL-2 gene is expressed in digestive cells. It is noteworthy that the other Pacific oyster lysozyme, CGL-1, was also transcribed in the same cells. Presence and expression of multiple lysozymes in the digestive diverticula suggest that CGL-1 and CGL-2 might play complementary roles in digestive organs.

Original languageEnglish
Pages (from-to)160-166
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume148
Issue number2
DOIs
Publication statusPublished - 2007 Oct

Keywords

  • CGL-1
  • CGL-2
  • Crassostrea gigas
  • Digestive tubules
  • In situ hybridization
  • Lysozymes
  • Pacific oyster
  • cDNA
  • i-type
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas'. Together they form a unique fingerprint.

  • Cite this