cDNA cloning and expression of human lactosylceramide synthase

Minoru Takizawa; Tomoko Nomura; Etsuji Wakisaka; Naonobu Yoshizuka; Junken Aoki; Hiroyuki Arai; Keizo Inoue; Michihiro Hattori; Noboru Matsuo

doi:10.1016/S1388-1981(99)00051-7

cDNA cloning and expression of human lactosylceramide synthase

Minoru Takizawa, Tomoko Nomura, Etsuji Wakisaka, Naonobu Yoshizuka, Junken Aoki, Hiroyuki Arai, Keizo Inoue, Michihiro Hattori, Noboru Matsuo

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. Copyright (C) 1999.

Original language	English
Pages (from-to)	301-304
Number of pages	4
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1438
Issue number	2
DOIs	https://doi.org/10.1016/S1388-1981(99)00051-7
Publication status	Published - 1999 May 18
Externally published	Yes

Keywords

Lactosylceramide
β1-4 Galactosyltransferase

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1388-1981(99)00051-7

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cDNA cloning and expression of human lactosylceramide synthase. / Takizawa, Minoru; Nomura, Tomoko; Wakisaka, Etsuji; Yoshizuka, Naonobu; Aoki, Junken; Arai, Hiroyuki; Inoue, Keizo; Hattori, Michihiro; Matsuo, Noboru.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1438, No. 2, 18.05.1999, p. 301-304.

Research output: Contribution to journal › Article › peer-review

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abstract = "Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. Copyright (C) 1999.",

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note = "Funding Information: This work was performed as part of an R&D Project within the Industrial Science and Technology Frontier Program, and was supported by NEDO (New Energy and Industrial Technology Development Organization).",

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AU - Takizawa, Minoru

AU - Nomura, Tomoko

AU - Wakisaka, Etsuji

AU - Yoshizuka, Naonobu

AU - Aoki, Junken

AU - Arai, Hiroyuki

AU - Inoue, Keizo

AU - Hattori, Michihiro

AU - Matsuo, Noboru

N1 - Funding Information: This work was performed as part of an R&D Project within the Industrial Science and Technology Frontier Program, and was supported by NEDO (New Energy and Industrial Technology Development Organization).

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N2 - Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. Copyright (C) 1999.

AB - Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. Copyright (C) 1999.

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cDNA cloning and expression of human lactosylceramide synthase

Abstract

Keywords

ASJC Scopus subject areas

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