cDNA cloning and expression of human lactosylceramide synthase

Minoru Takizawa, Tomoko Nomura, Etsuji Wakisaka, Naonobu Yoshizuka, Junken Aoki, Hiroyuki Arai, Keizo Inoue, Michihiro Hattori, Noboru Matsuo

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycolipids in mammals. We have isolated and sequenced the cDNA clone encoding human lactosylceramide synthase. The deduced amino acid sequence of the human lactosylceramide synthase showed 94.2% identity with rat lactosylceramide synthase. Northern blotting analysis revealed that lactosylceramide synthase mRNA was expressed in various tissues, with the highest level in brain and adrenal gland. Copyright (C) 1999.

Original languageEnglish
Pages (from-to)301-304
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1438
Issue number2
DOIs
Publication statusPublished - 1999 May 18
Externally publishedYes

Keywords

  • Lactosylceramide
  • β1-4 Galactosyltransferase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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