cDNA cloning and expression analysis of mouse zf9, a Kruppel-like transcription factor gene that is induced by adipogenic hormonal stimulation in 3T3-L1 cells

Hiroyuki Inuzuka, Hiroshi Wakao, Yasuhiko Masuho, Masa aki Muramatsu, Hideaki Tojo, Rika Nanbu-Wakao

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Using a differential hybridization method, we have cloned a zinc finger transcription factor gene whose expression was enhanced by adipogenic hormones in preadipocyte 3T3-L1 cells. Cloning of this gene revealed that it encodes a mouse homologue of rat Zf9 and human CPBP/GBF, previously identified as a wound-induced transcription factor and GC-rich binding protein, respectively. The mRNA for this clone consisted of 0.9 kb coding region and 3.2 kb long 3' untranslated region. Northern blot analysis revealed that it was ubiquitously expressed, among adult tissues, in which abundant expression was observed in lung, ovary and thymus. The transcript was transiently induced by different stimuli such as serum, cAMP and 12-O-tetradecanoylphorbol 13-acetate. Nuclear run-on and RNA synthesis inhibitor chase experiments indicated that the transient induction of the mRNA was regulated both at transcriptional and post-transcriptional levels. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1447
Issue number2-3
DOIs
Publication statusPublished - 1999 Oct 28
Externally publishedYes

Keywords

  • 3T3-L1 cell
  • Differential hybridization
  • Expression profile
  • Full length cDNA cloning
  • Kruppel-like zinc finger transcription factor

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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