Cdna and gene cloning of mammalian homologues of escheiuchia col! endonuclease III

S. Seki; A. H. Sarker; S. Lkeda; K. Akivjima; K. Imai; K. Tsutsni; Z. Bo; K. Vamanioto; A. Yasui; K. Kubo; M. C. Voshida

Cdna and gene cloning of mammalian homologues of escheiuchia col! endonuclease III

S. Seki, A. H. Sarker, S. Lkeda, K. Akivjima, K. Imai, K. Tsutsni, Z. Bo, K. Vamanioto, A. Yasui, K. Kubo, M. C. Voshida

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

To study mammalian enzymes for repair of oxidatively dam a cod DNA. < DNAs encoding homologues (inNthl and hNTHl) of Kxchrrtrkiti i-nli cndonuc-Icasc III (endo III; nth gene product) were isolated from moue arid human cDNA libraries, respectively, by screening with mouse cDN'A fragments am pli fied based on an information reported in KST Data Hases. inNthl and h.\ IHl cDNAs were 1025 and 1028 nucleotides long, and encode the cn/yme proteins consisting of iiOO and'304 ainino acid residues, respectively. Deduced amino acid sequences of both proteins exhibit significant homologios to thohf of cudo III and their oukaryotic homologues, including two motifs contesting, of a iielix hairpin-helix arid an iron-sulphur [4Fe-4S] cluster. niNthl niKNA (OT hNTHl niKNA) of approximately 1 kb was expressed in all mouse (or human) tissues analyzed. m.Nthl gene was cloned, and .shown to consist of (i exous spanning 6.09 kilobases. The boundaries between exon and intron follow the t.i'1 /A(! i u le.

Original language	English
Pages (from-to)	A1365
Journal	FASEB Journal
Volume	11
Issue number	9
Publication status	Published - 1997

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Genetics

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@article{238baa2c452b4e7896d0a57467ac9a39,

title = "Cdna and gene cloning of mammalian homologues of escheiuchia col! endonuclease III",

abstract = "To study mammalian enzymes for repair of oxidatively dam a cod DNA. < DNAs encoding homologues (inNthl and hNTHl) of Kxchrrtrkiti i-nli cndonuc-Icasc III (endo III; nth gene product) were isolated from moue arid human cDNA libraries, respectively, by screening with mouse cDN'A fragments am pli fied based on an information reported in KST Data Hases. inNthl and h.\ IHl cDNAs were 1025 and 1028 nucleotides long, and encode the cn/yme proteins consisting of iiOO and'304 ainino acid residues, respectively. Deduced amino acid sequences of both proteins exhibit significant homologios to thohf of cudo III and their oukaryotic homologues, including two motifs contesting, of a iielix hairpin-helix arid an iron-sulphur [4Fe-4S] cluster. niNthl niKNA (OT hNTHl niKNA) of approximately 1 kb was expressed in all mouse (or human) tissues analyzed. m.Nthl gene was cloned, and .shown to consist of (i exous spanning 6.09 kilobases. The boundaries between exon and intron follow the t.i'1 /A(! i u le.",

author = "S. Seki and Sarker, {A. H.} and S. Lkeda and K. Akivjima and K. Imai and K. Tsutsni and Z. Bo and K. Vamanioto and A. Yasui and K. Kubo and Voshida, {M. C.}",

year = "1997",

language = "English",

volume = "11",

pages = "A1365",

journal = "FASEB Journal",

issn = "0892-6638",

publisher = "FASEB",

number = "9",

}

TY - JOUR

T1 - Cdna and gene cloning of mammalian homologues of escheiuchia col! endonuclease III

AU - Seki, S.

AU - Sarker, A. H.

AU - Lkeda, S.

AU - Akivjima, K.

AU - Imai, K.

AU - Tsutsni, K.

AU - Bo, Z.

AU - Vamanioto, K.

AU - Yasui, A.

AU - Kubo, K.

AU - Voshida, M. C.

PY - 1997

Y1 - 1997

N2 - To study mammalian enzymes for repair of oxidatively dam a cod DNA. < DNAs encoding homologues (inNthl and hNTHl) of Kxchrrtrkiti i-nli cndonuc-Icasc III (endo III; nth gene product) were isolated from moue arid human cDNA libraries, respectively, by screening with mouse cDN'A fragments am pli fied based on an information reported in KST Data Hases. inNthl and h.\ IHl cDNAs were 1025 and 1028 nucleotides long, and encode the cn/yme proteins consisting of iiOO and'304 ainino acid residues, respectively. Deduced amino acid sequences of both proteins exhibit significant homologios to thohf of cudo III and their oukaryotic homologues, including two motifs contesting, of a iielix hairpin-helix arid an iron-sulphur [4Fe-4S] cluster. niNthl niKNA (OT hNTHl niKNA) of approximately 1 kb was expressed in all mouse (or human) tissues analyzed. m.Nthl gene was cloned, and .shown to consist of (i exous spanning 6.09 kilobases. The boundaries between exon and intron follow the t.i'1 /A(! i u le.

AB - To study mammalian enzymes for repair of oxidatively dam a cod DNA. < DNAs encoding homologues (inNthl and hNTHl) of Kxchrrtrkiti i-nli cndonuc-Icasc III (endo III; nth gene product) were isolated from moue arid human cDNA libraries, respectively, by screening with mouse cDN'A fragments am pli fied based on an information reported in KST Data Hases. inNthl and h.\ IHl cDNAs were 1025 and 1028 nucleotides long, and encode the cn/yme proteins consisting of iiOO and'304 ainino acid residues, respectively. Deduced amino acid sequences of both proteins exhibit significant homologios to thohf of cudo III and their oukaryotic homologues, including two motifs contesting, of a iielix hairpin-helix arid an iron-sulphur [4Fe-4S] cluster. niNthl niKNA (OT hNTHl niKNA) of approximately 1 kb was expressed in all mouse (or human) tissues analyzed. m.Nthl gene was cloned, and .shown to consist of (i exous spanning 6.09 kilobases. The boundaries between exon and intron follow the t.i'1 /A(! i u le.

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M3 - Article

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Cdna and gene cloning of mammalian homologues of escheiuchia col! endonuclease III

Abstract

ASJC Scopus subject areas

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