CD38 gene disruption inhibits the contraction induced by α-adrenoceptor stimulation in mouse aorta

Minori Mitsui-Saito, Ichiro Kato, Shin Takasawa, Hiroshi Okamoto, Teruyuki Yanagisawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

CD38 is an ectoenzyme with ADP-ribosyl cyclase and hydrolase activities, which synthesizes cyclic ADP-ribose from NAD and hydrolyzes cyclic ADP-ribose to ADP-ribose. It has been shown that cyclic ADP-ribose is a potent Ca 2+ mobilizing messenger in many cells. To know the physiological role of cyclic ADP-ribose in vascular smooth muscle, we examined the effects of various agonists in the aorta isolated from CD38 knockout (CD38-/-) mouse. Western blot analysis showed that CD38 protein was detected in the aorta isolated from wild-type (CD38+/+) mouse, but not from CD38 -/- mouse. In the aortae isolated from both CD38+/+ and CD38-/- mice, KCl, phenylephrine and norepinephrine induced concentration-dependent contraction. KCl produced similar concentration- dependent responses in the aortae from both CD38+/+ and CD38 -/- mice. Maximum force of contraction induced by KCl (65 mM) was same in the size. Phenylephrine- and norepinephrine-induced contractions were, however, significantly smaller in the aortae from CD38 -/- mice than in those from CD38+/+ mice. 5-Hydroxytryptamine, endothelin-1, caffeine and thapsigargin-induced contractions were not significantly different in these two aortae. These results suggest that CD38 gene disruption inhibits α-adrenoceptor-induced vascular contractions and cyclic ADP-ribose-mediated signal transduction system is committed in these responses.

Original languageEnglish
Pages (from-to)1325-1330
Number of pages6
JournalJournal of Veterinary Medical Science
Volume65
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Keywords

  • Aorta
  • CD38
  • Cyclic ADP-ribose
  • Mouse
  • Smooth muscle

ASJC Scopus subject areas

  • veterinary(all)

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