CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO

Tomonori Ishii, Kei Ohnuma, Akikazu Murakami, Naruhiko Takasawa, Seiji Kobayashi, Nam H. Dang, Stuart F. Schlossman, Chikao Morimoto

Research output: Contribution to journalArticlepeer-review

131 Citations (Scopus)

Abstract

CD26 is a T cell activation antigen that contains dipeptidyl peptidase IV activity and is known to bind adenosine deaminase. The mechanism by which CD26 costimulation potentiates T cell receptor-mediated T cell activation, leading to subsequent exertion of T cell effector function, is still not clearly defined. In this article, we demonstrate that CD26 localizes into lipid rafts, and targeting of CD26 to rafts is necessary for signaling events through CD26. Importantly, aggregation of CD26 by anti-CD26 mAb crosslinking also causes coaggregation of CD45 into rafts. Moreover, we show that CD26 directly binds to the cytoplasmic domain of CD45. Our results therefore indicate a mechanism whereby CD26 engagement promotes aggregation of lipid rafts and facilitates colocalization of CD45 to T cell receptor signaling molecules p56Lck, ZAP-70, and TCRζ, thereby enhancing protein tyrosine phosphorylation of various signaling molecules and subsequent interleukin-2 production.

Original languageEnglish
Pages (from-to)12138-12143
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number21
DOIs
Publication statusPublished - 2001 Oct 9

ASJC Scopus subject areas

  • General

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