TY - JOUR
T1 - Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defence-related genes in transgenic tobacco plants
AU - Takahashi, Hideki
AU - Shimamoto, Ko
AU - Ehara, Yoshio
PY - 1989/4/1
Y1 - 1989/4/1
N2 - In order to study possible functions of the inclusion body matrix protein (IBMP) encoded by gene VI of cauliflower mosaic virus (CaMV), the XbaI fragment containing the gene VI of a Japanese strain of CaMV (CaMV S-Japan) was transferred to tobacco plants by Ti mediated transformation. Eight out of 18 kanamycin resistant plants (40%) expressed detectable levels of IBMP. Those transgenic plants expressing IBMP produced leaves with light green color, and their growth was suppressed as compared with control plants. Symptom-like necrotic spots also appeared on the leaves and stems of the mature transgenic plants. Furthermore, in these transgenic plants, pathogenesis-related proteins 1a, 1b and 1c were highly expressed and the activity of 1,3-β-glucanase was increased up to eightfold. From these results, we concluded that expression of the IBMP is associated with symptom development.
AB - In order to study possible functions of the inclusion body matrix protein (IBMP) encoded by gene VI of cauliflower mosaic virus (CaMV), the XbaI fragment containing the gene VI of a Japanese strain of CaMV (CaMV S-Japan) was transferred to tobacco plants by Ti mediated transformation. Eight out of 18 kanamycin resistant plants (40%) expressed detectable levels of IBMP. Those transgenic plants expressing IBMP produced leaves with light green color, and their growth was suppressed as compared with control plants. Symptom-like necrotic spots also appeared on the leaves and stems of the mature transgenic plants. Furthermore, in these transgenic plants, pathogenesis-related proteins 1a, 1b and 1c were highly expressed and the activity of 1,3-β-glucanase was increased up to eightfold. From these results, we concluded that expression of the IBMP is associated with symptom development.
KW - Cauliflower mosaic virus gene VI
KW - Pathogenesis-related proteins
KW - Stress
KW - Symptom expression
KW - Transgenic tobacco plants
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U2 - 10.1007/BF00334355
DO - 10.1007/BF00334355
M3 - Article
AN - SCOPUS:0001259181
VL - 216
SP - 188
EP - 194
JO - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
JF - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
SN - 1617-4615
IS - 2-3
ER -