Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defence-related genes in transgenic tobacco plants

Hideki Takahashi, Ko Shimamoto, Yoshio Ehara

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    39 Citations (Scopus)

    Abstract

    In order to study possible functions of the inclusion body matrix protein (IBMP) encoded by gene VI of cauliflower mosaic virus (CaMV), the XbaI fragment containing the gene VI of a Japanese strain of CaMV (CaMV S-Japan) was transferred to tobacco plants by Ti mediated transformation. Eight out of 18 kanamycin resistant plants (40%) expressed detectable levels of IBMP. Those transgenic plants expressing IBMP produced leaves with light green color, and their growth was suppressed as compared with control plants. Symptom-like necrotic spots also appeared on the leaves and stems of the mature transgenic plants. Furthermore, in these transgenic plants, pathogenesis-related proteins 1a, 1b and 1c were highly expressed and the activity of 1,3-β-glucanase was increased up to eightfold. From these results, we concluded that expression of the IBMP is associated with symptom development.

    Original languageEnglish
    Pages (from-to)188-194
    Number of pages7
    JournalMGG Molecular & General Genetics
    Volume216
    Issue number2-3
    DOIs
    Publication statusPublished - 1989 Apr 1

    Keywords

    • Cauliflower mosaic virus gene VI
    • Pathogenesis-related proteins
    • Stress
    • Symptom expression
    • Transgenic tobacco plants

    ASJC Scopus subject areas

    • Genetics

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