Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defence-related genes in transgenic tobacco plants

Hideki Takahashi, Ko Shimamoto, Yoshio Ehara

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

In order to study possible functions of the inclusion body matrix protein (IBMP) encoded by gene VI of cauliflower mosaic virus (CaMV), the XbaI fragment containing the gene VI of a Japanese strain of CaMV (CaMV S-Japan) was transferred to tobacco plants by Ti mediated transformation. Eight out of 18 kanamycin resistant plants (40%) expressed detectable levels of IBMP. Those transgenic plants expressing IBMP produced leaves with light green color, and their growth was suppressed as compared with control plants. Symptom-like necrotic spots also appeared on the leaves and stems of the mature transgenic plants. Furthermore, in these transgenic plants, pathogenesis-related proteins 1a, 1b and 1c were highly expressed and the activity of 1,3-β-glucanase was increased up to eightfold. From these results, we concluded that expression of the IBMP is associated with symptom development.

Original languageEnglish
Pages (from-to)188-194
Number of pages7
JournalMGG Molecular & General Genetics
Volume216
Issue number2-3
DOIs
Publication statusPublished - 1989 Apr 1

Keywords

  • Cauliflower mosaic virus gene VI
  • Pathogenesis-related proteins
  • Stress
  • Symptom expression
  • Transgenic tobacco plants

ASJC Scopus subject areas

  • Genetics

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