@article{102d86b0dfcc4806b4d7ffca130724e9,
title = "Cationic liposome can interfere mRNA translation in an E. coli cell-free translation system",
abstract = "The effect of cationic liposome prepared from 1,2-dioleoyl-3-trimethyl- ammonium-propane (DOTAP) on the gene expression at the mRNA translation level was investigated using an E. coli cell-free translation system. DOTAP liposome at 3 mM inhibited the mRNA translation of green fluorescent protein (GFP), as indicated both by the fluorescence intensity of GFP and by SDS-PAGE analysis. The role of DOTAP liposome on the inhibition of mRNA translation was revealed that the cationic quaternary amine groups on the liposome surface can interact and neutralize the anionic phosphate groups on mRNA by an electrostatic interaction. mRNA molecules still existed without any degradation in the presence of DOTAP liposome although it could not be translated. These results clearly illustrated that the DOTAP liposome could knock down mRNA and silence its activity of translation in an E. coli cell-free system.",
keywords = "Cell free, GFP, Liposome, MRNA, Membrane stress biotechnology, Translation system",
author = "Bui, {Huong Thi} and Hiroshi Umakoshi and Keishi Suga and Tomoyuki Tanabe and Ngo, {Kien Xuan} and Toshinori Shimanouchi and Ryoichi Kuboi",
note = "Funding Information: The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology”. It was partly supported by a Grant-in-Aid for Scientific Research (Nos. 15206089 , 16686046 , 16760635 , 17656268 , 19656203 , 19656220 , and 20360350 ) from the Ministry of Education, Science, Sports, and Culture of Japan , a grant from the 21st Century COE program “Creation of Integrated EcoChemistry” and the Global COE program “Bio-Environmental Chemistry” of the Japan Society for the Promotion of Science (JSPS). The authors are also thankful for the useful discussion and comments from Prof. T. Tsuchido, Dr. Y. Matsumura, and Dr. J. Sakamoto (Graduate School of Chemistry, Materials, and Bioengineering, Kansai University, Suita, Japan), Dr. D. T. P. Thao (Kyoto University), Dr. D. N. Thai (Osaka University), Dr. N. B. Quoc and Dr. T. X. Hoat (Kobe University). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas hydrate Analyzing System of Osaka University. H.T. Bui acknowledges financial support from the Ministry of Education and Training, Vietnam (MOET) and the Japanese Society for Promotion of Science. ",
year = "2010",
month = oct,
doi = "10.1016/j.bej.2010.07.001",
language = "English",
volume = "52",
pages = "38--43",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
publisher = "Elsevier",
number = "1",
}