Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

Yusuke Hatakawa, Rina Nakamura, Motomi Konishi, Toshiyasu Sakane, Motoaki Saito, Toshifumi Akizawa

Research output: Contribution to journalArticlepeer-review

Abstract

We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.

Original languageEnglish
Article numbere02454
JournalHeliyon
Volume5
Issue number9
DOIs
Publication statusPublished - 2019 Sep
Externally publishedYes

Keywords

  • ANA/BTG3
  • Alzheimer's disease
  • Amyloid-β peptide
  • Analytical chemistry
  • Biochemistry
  • Biophysical chemistry
  • Biotechnology
  • Box A region
  • Catalytide
  • Chromatography
  • Developmental biology
  • Hydrolase activity
  • Molecular biology
  • Molecular modeling
  • Peptides
  • Structural biology
  • Tob/BTG

ASJC Scopus subject areas

  • General

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