Ca2+ channel activating action of maitotoxin in cultured brainstem neurons

Atsushi Kakizaki, Masami Takahashi, Hiroyuki Akagi, Eiichi Tachikawa, Toshifumi Yamamoto, Eiichi Taira, Tohru Yamakuni, Yasushi Ohizumi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The actions of maitotoxin were studied using cultured brainstem cells and adrenal chromaffin cells. Maitotoxin induced a profound increase in the Ca2+ influx into cultured brainstem cells after a brief lag period. The maitotoxin-induced Ca2+ influx was suppressed by various voltage-dependent Ca2+ channel blockers such as Co2+, Mn2+, verapamil and diltiazem. Maitotoxin-catecholamine release in brainstem cells initiated to increase after a lag period of about 1 min and the increase continued even at 4 min after treatment, while in the adrenal chromaffin cells the release started after an about 1-min lag period to attain a maximum within first 2-min and gradually decrease thereafter. These results suggest that maitotoxin acts on Ca2+ channels to increase the Ca2+ influx, accompanied by enhancement of catecholamine release in the brainstem cells with a different temporal profile from that in the adrenal chromaffin cells.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalEuropean Journal of Pharmacology
Volume536
Issue number3
DOIs
Publication statusPublished - 2006 May 1

Keywords

  • Adrenal chromaffin cell
  • Brainstem cell
  • Ca channel
  • Ca influx
  • Catecholamine release
  • Maitotoxin

ASJC Scopus subject areas

  • Pharmacology

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