TY - JOUR
T1 - Case report of rat true hermaphroditism
T2 - Colocalization of oocytes and granulosa and sertoli cells in the germinal cord
AU - Kai, Kiyonori
AU - Satoh, Norihiro
AU - Watanabe, Astushi
AU - Shiraiwa, Kazumi
AU - Sasano, Hironobu
AU - Furuhama, Kazuhisa
PY - 2003/5/1
Y1 - 2003/5/1
N2 - We describe a case of rat hermaphroditism with bilateral ovotestes. In a 7-week-old apparently male Sprague-Dawley rat, both testes were relatively small, and the right testis with a faint protrusion was somewhat round and small as compared with the left testis. Microscopically, the testes contained ovarian tissues within their tunica albugineas in conjunction with spermatogenesis in the seminiferous tubules. As bilateral changes, oocytes surrounded by granulosa-like cells were present in the seminiferous tubule-like germinal cord. Granulosa-like and Sertoli-like cells were layered together on the basal lamina, and theca interna-like cells were occasionally observed around the basal lamina. As unilateral changes, cystic dilatation of the germinal cords with eosinophilic fluid was seen in the lumen, and the theca interna-like cells appeared to be vacuolated. Immunohistochemically, the granulosa-like and Sertoli-like cells showed positive reactions for 3β-hydroxysteroid dehydrogenase (3β-HSD) and vimentin, respectively. Theca interna-like cells reacted positively to both 3β-HSD and cytochrome P-450 17α-hydroxylase. Ultrastructurally, the granulosa, Sertoli, and theca interna cells were also identified in the ovarian tissue. From these morphological characteristics, the male rat with bilateral ovotestes was diagnosed as true hermaphroditism.
AB - We describe a case of rat hermaphroditism with bilateral ovotestes. In a 7-week-old apparently male Sprague-Dawley rat, both testes were relatively small, and the right testis with a faint protrusion was somewhat round and small as compared with the left testis. Microscopically, the testes contained ovarian tissues within their tunica albugineas in conjunction with spermatogenesis in the seminiferous tubules. As bilateral changes, oocytes surrounded by granulosa-like cells were present in the seminiferous tubule-like germinal cord. Granulosa-like and Sertoli-like cells were layered together on the basal lamina, and theca interna-like cells were occasionally observed around the basal lamina. As unilateral changes, cystic dilatation of the germinal cords with eosinophilic fluid was seen in the lumen, and the theca interna-like cells appeared to be vacuolated. Immunohistochemically, the granulosa-like and Sertoli-like cells showed positive reactions for 3β-hydroxysteroid dehydrogenase (3β-HSD) and vimentin, respectively. Theca interna-like cells reacted positively to both 3β-HSD and cytochrome P-450 17α-hydroxylase. Ultrastructurally, the granulosa, Sertoli, and theca interna cells were also identified in the ovarian tissue. From these morphological characteristics, the male rat with bilateral ovotestes was diagnosed as true hermaphroditism.
KW - 3β-HSD
KW - Electron microscopy
KW - Hermaphroditism
KW - Immunohistochemistry
KW - Ovotestis
KW - P450c17
KW - Rat
KW - Vimentin
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U2 - 10.1080/01926230309755
DO - 10.1080/01926230309755
M3 - Article
C2 - 12746116
AN - SCOPUS:0038586506
VL - 31
SP - 290
EP - 294
JO - Toxicologic Pathology
JF - Toxicologic Pathology
SN - 0192-6233
IS - 3
ER -