Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

Masaki Ota, Hiromoto Watanabe, Yoshitaka Kato, Masaru Watanabe, Yoshiyuki Sato, Richard Lee Smith, Hiroshi Inomata

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Carotenoid production from highly CO2 tolerant microalga Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing was studied. Increasing temperature, increasing light intensity and decreasing CO2 and O2 gas concentrations enhanced growth rate under nitrate-rich conditions. Carotenoid content was insensitive to nitrate concentration, temperature and gas composition, but was greatly promoted by light intensity. Growth rate and carotenoid content had an optimum light intensity of ca. 120 μmol-photon · m-2s-1. Separation of two sample cultures was studied by applying supercritical fluid extraction with CO2 and 10 mol% ethanol co-solvent. Extraction yield of carotenoids was 90% with 10 mol% ethanol at 333 K and 30 MPa. Selectivity of a sample with less lipid content (12.9 wt%) was five-fold higher than that with higher lipid content (29.4 w%).

Original languageEnglish
Pages (from-to)2327-2335
Number of pages9
JournalJournal of Separation Science
Volume32
Issue number13
DOIs
Publication statusPublished - 2009 Sep 22

Keywords

  • CO
  • Co-solvent
  • Green alga
  • Microalgae
  • Supercritical fluid extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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