Carnosine protects against permanent cerebral ischemia in histidine decarboxylase knockout mice by reducing glutamate excitotoxicity

Yao Shen, Ping He, Yan ying Fan, Jian xiang Zhang, Hai jing Yan, Wei wei Hu, Hiroshi Ohtsu, Zhong Chen

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Recently, we showed that carnosine protects against NMDA-induced excitotoxicity in differentiated PC12 cells through a histaminergic pathway. However, whether the protective effect of the carnosine metabolic pathway also occurs in ischemic brain is unknown. Utilizing the model of permanent middle cerebral artery occlusion (pMCAO) in mice, we found that carnosine significantly improved neurological function and decreased infarct size in both histidine decarboxylase knockout and the corresponding wild-type mice to the same extent. Carnosine decreased the glutamate levels and preserved the expression of glutamate transporter-1 (GLT-1) but not the glutamate/aspartate transporter in astrocytes exposed to ischemia in vivo and in vitro. It suppressed the dissipation of ΔΨm and generation of mitochondrial reactive oxygen species (ROS) induced by oxygen-glucose deprivation in astrocytes. Furthermore, carnosine also decreased the mitochondrial ROS and reversed the decrease in GLT-1 induced by rotenone. These findings are the first to demonstrate that the mechanism of carnosine action in pMCAO may not be mediated by the histaminergic pathway, but by reducing glutamate excitotoxicity through the effective regulation of the expression of GLT-1 in astrocytes due to improved mitochondrial function. Thus, our study reveals a novel antiexcitotoxic agent in ischemic injury.

Original languageEnglish
Pages (from-to)727-735
Number of pages9
JournalFree Radical Biology and Medicine
Volume48
Issue number5
DOIs
Publication statusPublished - 2010 Mar 1

Keywords

  • Brain
  • Carnosine
  • Free radicals
  • Glutamate transporter-1
  • Ischemia
  • Mitochondrial function

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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