Carbon tetrachloride-induced hepatic and renal damages in rat: Inhibitory effects of cacao polyphenol

Teruo Miyazawa, Koichiro Suzuki, Kiyotaka Nakagawa, Takayuki Yamamoto, Taiki Miyazawa, Fumiko Kimura, Masanori Kamei

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Here, we investigated the protective effect of cacao polyphenol extract (CPE) on carbon tetrachloride (CCl4)-induced hepato-renal oxidative stress in rats. Rats were administered CPE for 7 days and then received intraperitoneal injection of CCl4. Two hours after injection, we found that CCl4 treatment significantly increased biochemical injury markers, lipid peroxides (phosphatidylcholine hydroperoxide (PCOOH) and malondialdehyde (MDA)) and decreased glutathione peroxidase activity in kidney rather than liver, suggesting that kidney is more vulnerable to oxidative stress under the present experimental conditions. CPE supplementation significantly reduced these changes, indicating that this compound has antioxidant properties against CCl4- induced oxidative stress. An inhibitory effect of CPE on CCl4-induced CYP2E1 mRNA degradation may provide an explanation for CPE antioxidant property. Together, these results provide quantitative evidence of the in vivo antioxidant properties of CPE, especially in terms of PCOOH and MDA levels in the kidneys of CCl4-treated rats.

Original languageEnglish
Pages (from-to)1669-1675
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number10
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • Cacao polyphenol
  • Carbon tetrachloride
  • Cytochrome P450 2E1(CYP2E1)
  • Glutathione peroxidase
  • Lipid peroxide

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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