Carbon Film Based Ring-Disk and Split-Disk Dual Electrodes as Detectors for Microbore Liquid Chromatography

Osamu Niwa, Masao Morita, Bruce P. Solomon, Peter T. Kissinger

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The electrochemical properties of carbon film based ring-disk and split-disk electrodes in small active volume (0.24-2 μL) radial flow cells were studied in order to employ them in conjunction with microbore liquid chromatography (LC) for determining catecholamines. The high conversion of dopamine (DA) at the disk of a ring-disk electrode makes the DA limiting current lower than the theoretical value at a low flow rate. The collection efficiency (CE) increases with decreasing flow rate and cell thickness, which is different from previously reported results. A high CE of 0.96 was achieved because of the narrow gap of 5 μm. On the other hand, a higher limiting current was observed with the split-disk than with a commercially available dual-disk electrode because of its efficient geometry for analyte collection. The cross talk of the DA after reacting at one of the electrode is only 2%. The ring-disk and split-disk electrodes were suitable as series and parallel electrochemical detectors for small bore LC. A detection limit of 103 fg was obtained for DA at the disk of the ring-disk because of the high signal in the thin-layer radial flow cell and the low noise level of the carbon film. In contrast, very slight interference was observed from neighboring electrodes when determining catecholamines at the split-disk.

Original languageEnglish
Pages (from-to)427-433
Number of pages7
JournalElectroanalysis
Volume8
Issue number5
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Keywords

  • Carbon film electrode
  • Liquid chromatography
  • Ring-disk electrode
  • Split-disk electrode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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