Carbon-Ag/AgCl probes for detection of cell activity in droplets

Kosuke Ino, Kaoru Ono, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.

Original languageEnglish
Pages (from-to)3832-3835
Number of pages4
JournalAnalytical Chemistry
Volume85
Issue number8
DOIs
Publication statusPublished - 2013 Apr 16

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Carbon-Ag/AgCl probes for detection of cell activity in droplets'. Together they form a unique fingerprint.

Cite this