Carbohydrate-restricted diet promotes skin senescence in senescence-accelerated prone mice

Qiming Wu, S. Shuang, Kazushi Yamamoto, Tsuyoshi Tsuduki

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


This study used senescence-accelerated prone mice (SAMP8) to examine the effects of a carbohydrate-restricted diet on aging and skin senescence, to determine how long-term carbohydrate restriction affects the aging process. Three-week-old male SAMP8 mice were divided into three groups after 1 week of preliminary feeding: one was given a controlled diet, the other was given a high-fat diet, and the third was given a carbohydrate-restricted diet. Ad libitum feeding was administered until the mice reached 50 weeks of age. Before the end of the test period, a grading test was used to evaluate visible aging in the mice. After the test period, serum and skin samples in mice were obtained and submitted for analysis. As a result, the grading test demonstrated that there was significant progression of visible aging in the carbohydrate-restricted group, as well as a decreased survival rate. Histological examination of the skin revealed that the epidermis and dermis in the carbohydrate-restricted group had become thinner. Analysis of the mechanisms involved demonstrated an increase in serum interleukin-6, aggravated skin senescence, inhibition of skin autophagy and activation of skin mTOR. Therefore, this study proved that a carbohydrate-restricted diet promoted skin senescence in senescence-accelerated mice.

Original languageEnglish
Pages (from-to)71-82
Number of pages12
Issue number1
Publication statusPublished - 2019 Feb 15


  • Aging
  • Autophagy
  • Carbohydrate-restricted diet
  • IL-6
  • Senescence-accelerated mice
  • mTOR

ASJC Scopus subject areas

  • Ageing
  • Gerontology
  • Geriatrics and Gerontology


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