Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients

Masashi Nakamura, Atsuo Kuramasu, Ichiro Nakashima, Kazuo Fujihara, Yasuto Itoyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of the present study was to detect antigenic proteins that react specifically with cerebrospinal fluid (CSF)-IgG from oligoclonal IgG bands (OB)-positive multiple sclerosis (MS) patients. To identify such antigenic proteins, we developed a rat brain proteome map using 2-DE and applied it to the immunoscreening of brain proteins that react with CSF-IgG but not with serum-IgG in OB-positive MS patients. After sequential MALDI-TOF mass spectrometry, eight proteins [two neuronal proteins (tubulin β-2 and γ enolase-2), HSP-1, Tpi-1 protein and cellular enzymes (creatine kinase, phosphopyruvate hydratase, triosephosphate isomerase and phosphoglycerate kinase-1)] were identified as candidate antigens in seven MS patients. Reactivity to tubulin was seen in Western blotting in four patients, and CSF-specific anti-tubulin IgG was detected in one patient. In addition, CSF-specific anti-gamma enolase IgG was found in another patient. These findings suggest that intrathecal immune responses may occur against a broad range of proteins in MS.

Original languageEnglish
Pages (from-to)681-687
Number of pages7
JournalProteomics - Clinical Applications
Volume1
Issue number7
DOIs
Publication statusPublished - 2007 Jul 1

Keywords

  • Cerebrospinal fluid
  • Multiple sclerosis
  • Neuron-specific enolase
  • Oligoclonal IgG bands
  • Tubulin

ASJC Scopus subject areas

  • Clinical Biochemistry

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