Campest-5-en-3-one, an oxidized derivative of campesterol, activates PPARα, promotes energy consumption and reduces visceral fat deposition in rats

Ikuo Ikeda, Rie Konno, Takeshi Shimizu, Takashi Ide, Nobuyuki Takahashi, Teruo Kawada, Koji Nagao, Nao Inoue, Teruyoshi Yanagita, Tadateru Hamada, Yae Morinaga, Hiroko Tomoyori, Katsumi Imaizumi, Kunio Suzuki

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Dietary campest-5-en-3-one (campestenone), an oxidized derivative of campesterol, significantly reduced visceral fat weight and the concentration of triacylglycerol in serum and liver of rats. Dietary campestenone dramatically increased the activities and the mRNA expressions of mitochondrial and peroxisomal enzymes involved in β-oxidation in the liver. Campestenone activated human peroxisome proliferator-activated receptor (PPAR) α as determined using the novel GAL4 ligand-binding domain chimera assay system with coactivator coexpression. In contrast, dietary campestenone reduced the activities and the mRNA expressions of enzymes involved in fatty acid synthesis, except for the malic enzyme. Dietary campestenone decreased the sterol regulatory element binding protein-1 (SREBP-1) mRNA level. Energy expenditure was significantly higher in the feeding of campestenone in rats. Dietary campestenone reduced hepatic cholesterol concentration and increased fecal excretion of neutral steroids originated from cholesterol. Lymphatic absorption of cholesterol was reduced by the coadministration of campestenone in rats cannulated in the thoracic duct. These observations suggest a possibility that campestenone has an ability to prevent coronary heart disease by improving obesity and abnormality of lipid metabolism.

Original languageEnglish
Pages (from-to)800-807
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1760
Issue number5
DOIs
Publication statusPublished - 2006 May

Keywords

  • Campest-5-en-3-one
  • Campesterol
  • PPARα
  • SREBP-1c
  • Visceral fat

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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