CaMKII phosphorylates serine 10 of p27 and confers apoptosis resistance to HeLa cells

Ryutaro Kajihara, Shota Fukushige, Norifumi Shioda, Kano Tanabe, Koji Fukunaga, Seiji Inui

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Protein phosphatase (PP) 6 is a serine threonine phosphatase which belongs to the PP2A subfamily of protein phosphatases. PP6 has been implicated in the control of apoptosis. A dominant negative form PP6 (DN-PP6) mutant cDNA was prepared and transfected into HeLa cells to investigate the regulation of apoptosis. HeLa cells expressing DN-PP6 showed increased resistance to apoptosis induced by TNF and cycloheximide. CaMKII phosphorylation and the expression of p27 were increased in DN-PP6 transfectants. Transient expression or activation of CaMKII increased the expression of p27. Furthermore, CaMKII phosphorylated serine 10 of p27, which induces the translocation of p27 from nucleus to cytoplasm and increases the stability of p27. Overexpression of wild type but not the S10A mutant p27 cDNA increased the expression of Bcl-xL and conferred apoptosis resistance to HeLa cells. These results indicated that PP6 and CaMKII regulated apoptosis by controlling the expression level of p27.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalBiochemical and biophysical research communications
Volume401
Issue number3
DOIs
Publication statusPublished - 2010 Oct 22

Keywords

  • Apoptosis
  • CaMKII
  • P27
  • Protein phosphatase 6

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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