A heat-stable factor with properties similar to those of calmodulin was found in the fraction containing Ca2+-dependent cyclic AMP phosphodiesterase of Escherichia coli. The factor activated such enzymes as cyclic nucleotide phosphodiesterase of bovine brain, (Ca2+,Mg2+)ATPase of human erythrocyte menbrane and myosin light chain kinase of rabbit myometrium in a Ca2+-dependent fashion with an apparent Ka of 5 × 10-5 M. The factor and brain calmodulin had no effect on the phosphodiesterase of E. coli. It may be concluded that calmodulin or a calmodulin-like protein occurs in prokaryotes.
|Number of pages||5|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1981 Feb 12|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology