Calcium signalling in and around the nuclear envelope

J. Gerasimenko, Y. Maruyama, A. Tepikin, O. H. Petersen, O. Gerasimenko

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

We have compared calcium mobilization by Ins(1,4,S)P3 (IP3), cADP-ribose (cADPR) and nicotinic acidadenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca2+ uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that all calcium messengers induce Ca2+ release from the nuclear envelope. Pre-treatment of nuclei with thapsigargin completely abolished the responses to the calcium messengers, indicating that Ca2+ stores in isolated nuclei are thapsigargin-sensitive. Using different pharmacological tools, we show that Ca2+ release from pancreatic nuclei is unlikely to occur from stores other than those with endoplasmic reticulum characteristics, we conclude that all three calcium messengers can release Ca2+ from pancreatic acinar nuclear stores, as previously shown for IP3 and cADPR. It would appear that NAADP releases Ca2+ from the same IP3- and cADPR-sensitive stores with endoplasmic reticulum characteristics.

Original languageEnglish
Pages (from-to)76-78
Number of pages3
JournalBiochemical Society Transactions
Volume31
Issue number1
DOIs
Publication statusPublished - 2003 Feb

Keywords

  • Inositol 1,4,5-trisphosphate
  • NAADP
  • cADPR

ASJC Scopus subject areas

  • Biochemistry

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