Abstract
We have compared calcium mobilization by Ins(1,4,S)P3 (IP3), cADP-ribose (cADPR) and nicotinic acidadenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca2+ uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that all calcium messengers induce Ca2+ release from the nuclear envelope. Pre-treatment of nuclei with thapsigargin completely abolished the responses to the calcium messengers, indicating that Ca2+ stores in isolated nuclei are thapsigargin-sensitive. Using different pharmacological tools, we show that Ca2+ release from pancreatic nuclei is unlikely to occur from stores other than those with endoplasmic reticulum characteristics, we conclude that all three calcium messengers can release Ca2+ from pancreatic acinar nuclear stores, as previously shown for IP3 and cADPR. It would appear that NAADP releases Ca2+ from the same IP3- and cADPR-sensitive stores with endoplasmic reticulum characteristics.
Original language | English |
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Pages (from-to) | 76-78 |
Number of pages | 3 |
Journal | Biochemical Society Transactions |
Volume | 31 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2003 Feb |
Keywords
- Inositol 1,4,5-trisphosphate
- NAADP
- cADPR
ASJC Scopus subject areas
- Biochemistry