Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): Comparison of histamine-induced calcium mobilization

Kazuhiko Yanai, Kazutaka Maeyama, Norimichi Nakahata, Hironori Nakanishi, Takehiko Watanabe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Intracellular free Ca2+ concentration ([Ca2+]i) was monitored in monolayer of 1321 N1 astrocytoma cells by using a fluorescent Ca2+ indicator fura-2. Endothelin-1 (ET-1), endothelin-2 (ET-2), and sarafotoxin Sb6 (SRTX) increased [Ca2+]i from 56 ± 6 nM to 360 ± 82, 120 ± 51, 143 ± 29 nM, respectively, immediately after their addition to the perfusate with maximum response of more than 0.1 μM of peptides. Endothelin-3 (< 1 μM) did not affect [Ca2+]i. The increase in [Ca2+]i in response to either ET-1, ET-2, or SRTX could be almost completely inhibited by pretreating cells with ET-1 or ET-2. The homologous desensitization of [Ca2+]i induced by ETs and SRTX is in good agreement with their affinity toward an ETA receptors. The responses in [Ca2+]i by ETs and SRTX were not affected in the desensitized state induced by the pretreatment of histamine in the presence of extracellular Ca2+. However, the response in [Ca2+]i by ETs and SRTX were reduced in the desensitized state induced by pretreatment of histamine in the absence of extracellular Ca2+. These results indicate that depletion of the intracellular Ca2+ stores is responsible for the heterologous desensitization between ETs and histamine.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume346
Issue number1
DOIs
Publication statusPublished - 1992 Jul

Keywords

  • Astrocytoma cell line
  • Desensitization
  • Endothelins
  • Histamine
  • Intracellular Ca

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): Comparison of histamine-induced calcium mobilization'. Together they form a unique fingerprint.

Cite this