Calcitonin in human odontoclasts regulates root resorption activity via protein kinase A

Keisuke Takada, Hiroshi Kajiya, Hidefumi Fukushima, Fujio Okamoto, Wataru Motokawa, Koji Okabe

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Calcitonin is a known inhibitor of osteoclastic bone resorption, but it remains uncertain whether calcitonin also regulates human odontoclastic activity, particularly during the physiological process of root resorption. In this study, we examined the expression of calcitonin receptors in human odontoclasts and the effect of calcitonin on root resorption, using immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Actin-ring formation was used to assess cytostructural changes during resorption activity. Our results show that calcitonin receptors are expressed in human odontoclasts freshly isolated from deciduous teeth of the periodontal region. Calcitonin inhibited actin-ring formation and resorption activity. This calcitonin-induced inhibition was mimicked by forskolin and dibutyryladenosine 3′,5′-cyclic monophosphate (db-cAMP), which are protein kinase A (PKA) activators, but not by phorbol 12-myristate 13-acetate, a protein kinase C activator. Pretreatment with adenosine 3′,5′-cyclic monophosphothioate Rp diastereomer (Rp-cAMPS), a PKA inhibitor, suppressed the calcitonin-induced inhibition of actin-ring formation. These results indicate that calcitonin receptor activation suppresses odontoclastic root resorption via PKA, a signaling pathway different from that in human osteoclasts.

Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalJournal of Bone and Mineral Metabolism
Volume22
Issue number1
DOIs
Publication statusPublished - 2004 Feb 9

Keywords

  • Calcitonin
  • Odontoclasts
  • Physiological root resorption
  • Protein kinase A

ASJC Scopus subject areas

  • Endocrinology

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