Calcineurin/NFAT pathway: A novel regulator of parturition

Chisa Tabata, Kazuhide Ogita, Keisuke Sato, Hitomi Nakamura, Zhang Qing, Hidenori Negoro, Keiichi Kumasawa, Kumiko Temma-Asano, Tateki Tsutsui, Katsuhiko Nishimori, Tadashi Kimura

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Problem: The oxytocin (OT)-oxytocin receptor (OTR) system plays an important role in mammalian parturition. However, we found OTR-deficient (OTRKO) mice are fertile and deliver at term without birth defects, thus alternative pathways inducing parturition can be hypothesized. Method of study: We tested the gene expression profile of OTRKO mice using suppressive subtractive hybridization, and focused on the calcineurin/nuclear factor of activated T cells (NFAT) pathway. We examined the expression and localization of this pathway in mouse parturition. Results: Calcineurin and NFATc1 were detected in the decidua of pregnant uteri at term using immunohistochemistry (IHC). We identified higher activation levels of NFATc1 in wild type (WT) than in OTRKO mice and increased calcineurin A and NFATc1 mRNA levels during pregnancy. Moreover, injection of FK506, the inhibitor of this pathway, prolonged the delivery of the first pup. Conclusions: Our findings suggested thatthe calcineurin/NFAT pathway might play a substantial role in initiation of labor.

    Original languageEnglish
    Pages (from-to)44-50
    Number of pages7
    JournalAmerican Journal of Reproductive Immunology
    Volume62
    Issue number1
    DOIs
    Publication statusPublished - 2009

    Keywords

    • FK506
    • Oxytocin
    • Oxytocin receptor-deficient mouse
    • Suppression subtractive hybridization

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Reproductive Medicine
    • Obstetrics and Gynaecology

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