Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner

Yohei Sasagawa, Mieko Otani, Nahoko Higashitani, Atsushi Higashitani, Ken Sato, Teru Ogura, Kunitoshi Yamanaka

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and meiotic and mitotic progression. However, its cellular functions in development have not yet been clarified. Here, we present data that p97 is involved in the switch from spermatogenesis to oogenesis in the germline of the C. elegans hermaphrodite. We found that the cdc-48.1 deletion mutant produced less sperm than the wild type and thus showed a decreased brood size. The cdc-48.1 mutation suppressed the sperm-overproducing phenotypes of fbf-1 and fem-3(gf) mutants. In addition, the p97/CDC-48-UFD-1-NPL-4 complex interacted with the E3 ubiquitin ligase CUL-2 complex via NPL-4 binding to Elongin C. Furthermore, TRA-1A, which is the terminal effector of the sex determination pathway and is regulated by CUL-2-mediated proteolysis, accumulated in the cdc-48.1 mutant. Proteasome activity was also required for the brood size determination and sperm-oocyte switch. Our results demonstrate that the C. elegans p97/CDC-48-UFD-1-NPL-4 complex controls the sperm-oocyte switch by regulating CUL-2-mediated TRA-1A proteasome degradation.

Original languageEnglish
Pages (from-to)3663-3672
Number of pages10
JournalJournal of cell science
Volume122
Issue number20
DOIs
Publication statusPublished - 2009 Oct 15

Keywords

  • AAA family
  • C. elegans
  • P97/Cdc48p
  • Sex determination
  • TRA-1
  • Ubiquitin ligase

ASJC Scopus subject areas

  • Cell Biology

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