Caenorhabditis elegans Aurora A kinase is required for the formation of spindle microtubules in female meiosis

Eisuke Sumiyoshi, Yuma Fukata, Satoshi Namai, Asako Sugimoto

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    In many animals, female meiotic spindles are assembled in the absence of centrosomes, the major microtubule (MT)-organizing centers. How MTs are formed and organized into meiotic spindles is poorly understood. Here we report that, in Caenorhabditis elegans, Aurora A kinase/AIR-1 is required for the formation of spindle microtubules during female meiosis. When AIR-1 was depleted or its kinase activity was inhibited in C. elegans oocytes, although MTs were formed around chromosomes at germinal vesicle breakdown (GVBD), they were decreased during meiotic prometaphase and failed to form a bipolar spindle, and chromosomes were not separated into two masses. Whereas AIR-1 protein was detected on and around meiotic spindles, its kinase-active form was concentrated on chromosomes at prometaphase and on interchromosomal MTs during late anaphase and telophase. We also found that AIR-1 is involved in the assembly of short, dynamic MTs in the meiotic cytoplasm, and these short MTs were actively incorporated into meiotic spindles. Collectively our results suggest that, after GVBD, the kinase activity of AIR-1 is continuously required for the assembly and/or stabilization of female meiotic spindle MTs.

    Original languageEnglish
    Pages (from-to)4187-4196
    Number of pages10
    JournalMolecular biology of the cell
    Volume26
    Issue number23
    DOIs
    Publication statusPublished - 2015 Nov 15

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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