C9-R95X polymorphism in patients with neovascular age-related macular degeneration

Koji M. Nishiguchi, Tetsuhiro R. Yasuma, Daisuke Tomida, Makoto Nakamura, Kohei Ishikawa, Masato Kikuchi, Yuhsuke Ohmi, Toshimitsu Niwa, Nobuyuki Hamajima, Koichi Furukawa, Hiroko Terasaki

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

PURPOSE. A non-sense mutation at codon 95 in the gene encoding complement factor C9 (C9-R95X) is found most frequently among Japanese. The authors investigated the association between C9-R95X and Japanese patients with neovascular agerelated macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV). METHODS. The presence of the C9-R95X polymorphism was assessed by direct sequencing in Japanese patients with either PCV (n = 105) or neovascular AMD (n = 198) and 396 control subjects. Multivariate regression analyses were conducted. Photocoagulation was applied in the eyes of mice with a heterozygous defect in the C3 gene and control wild-type mice. Photocoagulation was also applied to wild-type mice before either anti-C9 antibody or isotype IgG was injected into the eyes. The eyes were collected later for measurement of vascular endothelial growth factor (VEGF) and histological evaluation of choroidal neovascularization (CNV). RESULTS. The frequency of those with one or two C9-R95X variants was lower in neovascular AMD (2.02%) than in PCV (5.71%) and controls (6.05%). The presence of C9-R95X conferred a 4.7-fold reduction (95% confidence interval, 1.2-18.1; P = 0.021) in the risk for neovascular AMD after adjusting for the major AMD risk factors. A heterozygous defect in the C3 gene was associated with the reduced growth of laser-induced CNV, as was intraocular injection of anti-C9 antibody. This reduced CNV growth was accompanied by a decreased level of secreted VEGF in the intraocular fluid. CONCLUSIONS. These findings support the notion that the haploinsufficiency of C9, a terminal complement complex component, engenders reduced intraocular secretion of VEGF and decreased risk for CNV development.

Original languageEnglish
Pages (from-to)508-512
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number1
DOIs
Publication statusPublished - 2012 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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