TY - JOUR
T1 - C-type natriuretic peptide in the human central nervous system
T2 - Distribution and molecular form
AU - Totsune, Kazuhito
AU - Takahashi, Kazuhiro
AU - Ohneda, Makoto
AU - Itoi, Keiichi
AU - Murakami, Osamu
AU - Mouri, Toraichi
PY - 1994/1
Y1 - 1994/1
N2 - The distribution and molecular form of C-type natriuretic peptide (CNP) in the human central nervous system were studied with a specific radioimmunoassay for CNP-22. Immunoreactive (IR-) CNP was detectable in all regions of the brain examined (cerebral cortex, thalamus, hypothalamus, pons, and cerebellum) (0.21-0.81 pmol/g wet tissue, n = 4). The highest concentration of IR-CNP was found in the spinal cord at 1.83 ± 0.13 pmol/g wet tissue (mean ± SD, n = 3). Reversed-phase high performance liquid chromatography revealed a major peak migrating at the position corresponding to synthetic human CNP-53 and minor peaks comigrating with synthetic CNP-22 and the methionine-oxidized form of CNP-22, respectively. These findings suggest that IR-CNP is widely present in the human central nervous system mainly in a high molecular weight form as the major component and in the molecular form of CNP-22 as the minor component.
AB - The distribution and molecular form of C-type natriuretic peptide (CNP) in the human central nervous system were studied with a specific radioimmunoassay for CNP-22. Immunoreactive (IR-) CNP was detectable in all regions of the brain examined (cerebral cortex, thalamus, hypothalamus, pons, and cerebellum) (0.21-0.81 pmol/g wet tissue, n = 4). The highest concentration of IR-CNP was found in the spinal cord at 1.83 ± 0.13 pmol/g wet tissue (mean ± SD, n = 3). Reversed-phase high performance liquid chromatography revealed a major peak migrating at the position corresponding to synthetic human CNP-53 and minor peaks comigrating with synthetic CNP-22 and the methionine-oxidized form of CNP-22, respectively. These findings suggest that IR-CNP is widely present in the human central nervous system mainly in a high molecular weight form as the major component and in the molecular form of CNP-22 as the minor component.
KW - Chromatography
KW - Human brain
KW - Radioimmunoassay
KW - Spinal cord
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U2 - 10.1016/0196-9781(94)90167-8
DO - 10.1016/0196-9781(94)90167-8
M3 - Article
C2 - 8015979
AN - SCOPUS:0028196786
VL - 15
SP - 37
EP - 40
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 1
ER -