TY - JOUR
T1 - C-type natriuretic peptide in the human central nervous system
T2 - Distribution and molecular form
AU - Totsune, Kazuhito
AU - Takahashi, Kazuhiro
AU - Ohneda, Makoto
AU - Itoi, Keiichi
AU - Murakami, Osamu
AU - Mouri, Toraichi
N1 - Funding Information:
The study was supported in part by a research grant from the Miyagi Prefecture Kidney Association. The authors are grateful to Dr. H. Sasano, Second Department of Pathology, Tohoku University School of Medicine, and Dr. Y. Tachibana, Takeda Hospital, for providing the post-mortem human brain tissues, and to Ms. ltoh for her secretarial assistance.
PY - 1994/1
Y1 - 1994/1
N2 - The distribution and molecular form of C-type natriuretic peptide (CNP) in the human central nervous system were studied with a specific radioimmunoassay for CNP-22. Immunoreactive (IR-) CNP was detectable in all regions of the brain examined (cerebral cortex, thalamus, hypothalamus, pons, and cerebellum) (0.21-0.81 pmol/g wet tissue, n = 4). The highest concentration of IR-CNP was found in the spinal cord at 1.83 ± 0.13 pmol/g wet tissue (mean ± SD, n = 3). Reversed-phase high performance liquid chromatography revealed a major peak migrating at the position corresponding to synthetic human CNP-53 and minor peaks comigrating with synthetic CNP-22 and the methionine-oxidized form of CNP-22, respectively. These findings suggest that IR-CNP is widely present in the human central nervous system mainly in a high molecular weight form as the major component and in the molecular form of CNP-22 as the minor component.
AB - The distribution and molecular form of C-type natriuretic peptide (CNP) in the human central nervous system were studied with a specific radioimmunoassay for CNP-22. Immunoreactive (IR-) CNP was detectable in all regions of the brain examined (cerebral cortex, thalamus, hypothalamus, pons, and cerebellum) (0.21-0.81 pmol/g wet tissue, n = 4). The highest concentration of IR-CNP was found in the spinal cord at 1.83 ± 0.13 pmol/g wet tissue (mean ± SD, n = 3). Reversed-phase high performance liquid chromatography revealed a major peak migrating at the position corresponding to synthetic human CNP-53 and minor peaks comigrating with synthetic CNP-22 and the methionine-oxidized form of CNP-22, respectively. These findings suggest that IR-CNP is widely present in the human central nervous system mainly in a high molecular weight form as the major component and in the molecular form of CNP-22 as the minor component.
KW - Chromatography
KW - Human brain
KW - Radioimmunoassay
KW - Spinal cord
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U2 - 10.1016/0196-9781(94)90167-8
DO - 10.1016/0196-9781(94)90167-8
M3 - Article
C2 - 8015979
AN - SCOPUS:0028196786
VL - 15
SP - 37
EP - 40
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 1
ER -