C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells

Kazuhiko Okumura, Akifumi Itoh, Emiko Isogai, Kimiharu Hirose, Yoichiro Hosokawa, Yoshihiro Abiko, Takatoshi Shibata, Michimasa Hirata, Hiroshi Isogai

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99 Citations (Scopus)

Abstract

Mammalian myeloid and epithelial cells express many antimicrobiotic peptides that contribute to innate host defense against invading microbes. In the present study, a 27-mer peptide of the C-terminal domain (hCAP18 109-135) and analogs of the antimicrobial peptide human cathelicidin LL-37/human cationic antimicrobial protein 18 (hCAP18) were examined for tumoricidal activity. In vitro results showed that hCAP18109-135 induced apoptosis in human oral squamous cell carcinoma (OSCC), SAS-H1 cells. The hCAP18109-135 induced mitochondrial depolarization and apoptosis in SAS-H1 cells, but not in healthy human gingival fibroblasts (HGF) and human keratinocyte line HaCaT cells. Caspases were not activated during hCAP18 109-135-induced apoptosis in SAS-H1 cells. The results indicate that hCAP18109-135 may induce caspase-independent apoptosis in OSCC but not in normal cells. hCAP18109-135 can therefore be a useful anti-tumor therapeutic agent in the treatment of OSCC.

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalCancer Letters
Volume212
Issue number2
DOIs
Publication statusPublished - 2004 Aug 30

Keywords

  • Apoptosis
  • C-terminal domain of hCAP18 peptides
  • Human oral squamos cell carcinoma cells
  • Hydrophobicity
  • Mitochondrial membrane potential

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Okumura, K., Itoh, A., Isogai, E., Hirose, K., Hosokawa, Y., Abiko, Y., Shibata, T., Hirata, M., & Isogai, H. (2004). C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells. Cancer Letters, 212(2), 185-194. https://doi.org/10.1016/j.canlet.2004.04.006