c-Maf is essential for the F4/80 expression in macrophages in vivo

Megumi Nakamura, Michito Hamada, Kazuteru Hasegawa, Manabu Kusakabe, Hirona Suzuki, David R. Greaves, Takashi Moriguchi, Takashi Kudo, Satoru Takahashi

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

c-Maf, which is one of the large Maf transcription factors, can bind to Maf recognition element (MARE) and activates transcription of target genes. Although c-Maf is expressed in macrophages and directly regulates the expression of interleukin-10, detailed information regarding its function in the null mutant phenotype of tissue macrophages remain unknown. In this study, we demonstrated that c-Maf is specifically expressed in the F4/80 positive fetal liver and adult macrophages. The expression of F4/80, which is a tissue macrophage-specific seven trans-membrane receptor, was dramatically suppressed in the c-Maf-deficient macrophage, whereas the expression of Mac-1 was not affected, suggesting that c-Maf is not necessary for the lineage commitment of macrophages. Luciferase reporter and EMSA showed that c-Maf directly regulates the expression of F4/80 by interacting with the half-MARE site of the F4/80 promoter. These results suggest that c-Maf is required for the F4/80 expression in macrophages in vivo.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalGene
Volume445
Issue number1-2
DOIs
Publication statusPublished - 2009 Sep 15

Keywords

  • F4/80
  • Large Maf
  • Macrophage
  • c-Maf

ASJC Scopus subject areas

  • Genetics

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