c-kit gene expression in CD7-positive acute lymphoblastic leukemia: Close correlation with expression of myeloid-associated antigen CD13

Kazuhiro Nishii, Kenkichi Kita, Hiroshi Miwa, Keiki Kawakami, Kazunori Nakase, Masahiro Masuya, Norihisa Morita, Serdar B. Omay, Noriko Otsuji, Manabu Fukumoto, Shigeru Shirakawa

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Abstract

Expression of human c-kit proto-oncogene and interleukin-7 receptor (IL-7R) in acute lymphoblastic leukemia (ALL) cells expressing CD7 was examined by Northern-blot analysis and reversed transcription polymerase chain reaction (RT-PCR) assay in relation to the phenotypes. Leukemic cells from four out of 12 CD7+ ALL patients, all of which fulfilled the criteria of ALL in the FAB classification, expressed c-kit genes. Surface CD3 (sCD3) was absent in all of these cases, while cytoplasmic CD3 (cCD3) was found in the two sCD3- cases. CDSe transcripts were detected in one of the sCD3- cCD3- cases. IL-7R genes were transcribed in the three cases with c-kit gene expression. In addition, there was a good correlation between c-kit gene expression and myeloid associated antigen CD13 positivity of the leukemic cells. None of the patients with c-kit gene expression had mediastinal tumor. Our results show that leukemic cells in a proportion of CD7+ ALL express receptors for cytokines that are secreted by bone marrow stromal cells. Ligands for c-kit genes and IL-7 could play an important role for the regulation of proliferation and differentiation of T-cell progenitors in bone marrow.

Original languageEnglish
Pages (from-to)662-668
Number of pages7
JournalLeukemia
Volume6
Issue number7
Publication statusPublished - 1992 Jul

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Nishii, K., Kita, K., Miwa, H., Kawakami, K., Nakase, K., Masuya, M., Morita, N., Omay, S. B., Otsuji, N., Fukumoto, M., & Shirakawa, S. (1992). c-kit gene expression in CD7-positive acute lymphoblastic leukemia: Close correlation with expression of myeloid-associated antigen CD13. Leukemia, 6(7), 662-668.