TY - JOUR
T1 - BSF‐2/IL‐6 does not augment lg secretion but stimulates proliferation in myeloma cells
AU - Tanabe, Osamu
AU - Kawano, Michio
AU - Tanaka, Hideo
AU - Iwato, Koji
AU - Asaoku, Hideki
AU - Ishikawa, Hideaki
AU - Nobuyoshi, Masaharu
AU - Hirano, Toshio
AU - Kishimoto, Tadamitsu
AU - Kuramoto, Atsushi
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Human myeloma cells were highly purified from bone marrow aspirates of 21 patients with advanced immunoglobulin G (IgG)‐type multiple myeloma. B‐cell stimulatory factor 2 (BSF‐2)/interleukin‐6 (IL‐6) was originally characterized as a cytokine that can enhance immunoglobulin secretion from activated normal B cells and increase the expression of secretory‐type Ig mRNA in these B cells, but that does not augment proliferation of activated B cells. However, recombinant IL‐6 (rIL‐6) could not enhance M‐protein (IgG) secretion in freshly isolated myeloma cells in vitro but could augment proliferation of myeloma cells, although myeloma cells constitutively expressed IL‐6 receptors. Furthermore, expression of secretory‐type IgG (γ‐chain) mRNA in myeloma cells was not changed in the presence of IL‐6. These results show that IL‐6 is not an enhancing factor in Ig secretion from myeloma cells, and thus signal transduction through IL‐6 in myeloma cells may be altered as opposed to activated B cells.
AB - Human myeloma cells were highly purified from bone marrow aspirates of 21 patients with advanced immunoglobulin G (IgG)‐type multiple myeloma. B‐cell stimulatory factor 2 (BSF‐2)/interleukin‐6 (IL‐6) was originally characterized as a cytokine that can enhance immunoglobulin secretion from activated normal B cells and increase the expression of secretory‐type Ig mRNA in these B cells, but that does not augment proliferation of activated B cells. However, recombinant IL‐6 (rIL‐6) could not enhance M‐protein (IgG) secretion in freshly isolated myeloma cells in vitro but could augment proliferation of myeloma cells, although myeloma cells constitutively expressed IL‐6 receptors. Furthermore, expression of secretory‐type IgG (γ‐chain) mRNA in myeloma cells was not changed in the presence of IL‐6. These results show that IL‐6 is not an enhancing factor in Ig secretion from myeloma cells, and thus signal transduction through IL‐6 in myeloma cells may be altered as opposed to activated B cells.
KW - IL‐6
KW - Ig secretion
KW - multiple myeloma
KW - myeloma cell proliferation
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U2 - 10.1002/ajh.2830310408
DO - 10.1002/ajh.2830310408
M3 - Article
C2 - 2787116
AN - SCOPUS:0024337602
VL - 31
SP - 258
EP - 262
JO - American Journal of Hematology
JF - American Journal of Hematology
SN - 0361-8609
IS - 4
ER -