TY - JOUR
T1 - Broad spectrum anti-RNA virus activities of titanium and vanadium substituted polyoxotungstates
AU - Shigeta, Shibro
AU - Mori, Shuichi
AU - Kodama, Eiichi
AU - Kodama, Junko
AU - Takahashi, Kazuo
AU - Yamase, Toshihiro
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Seven polyoxotungstates substituted with vanadium or titanium atoms were examined for their activity against Flaviviridae (Dengue fever virus, DFV), Orthomyxoviridae (influenza virus type A, fluV-A), Paramyxoviridae (respiratory syncytial virus, RSV, parainfluenza virus type 2, PfluV-2 and canine distemper virus, CDV) and Lentiviridae (human immunodeficiency virus type 1, HIV-1) families. Among the seven polyoxotungstates examined, PM-43 {K5[SiVW11O40]}, PM-47 {K7[BVW11O40]}, and PM-1001 [K10Na(VO)3(SbW9O33) 2]26H2O contained vanadium. PM-1002 had the same core structure of (VO)3(SbW9O33)2 as PM-1001; however, three V atoms of PM-1001 consisted of two VIV and one VV and those of PM-1002 consisted of three VIV. On the other hand, PM-518 {[Et2NH2]7[PTi2W 10O40]}, PM-520 [Pri2NH2]5[PTiW11O40] and PM-523 [PriNH3]6H[PTi2W10O 38(O2)2]H2O all contained titanium. All compounds showed broad spectrum antiviral activity against all viruses examined except for PMs-43, -518 and -523 which did not exhibit inhibitory activity at ≥50μM against PfluV-2, CDV and DFV, respectively. All compounds were inhibitory against HIV replication at an EC50 of less than 2.0μM. Among them, PMs-1001 and -1002 showed the most potent inhibition. The compounds were not toxic for MDCK, HEp-2 and Vero cells at a concentration of 200μM. For the exponentially growing MT-4 cells, the vanadium containing polyoxometalates (PMs-43, 47, 1001, 1002) showed toxicity at concentrations between 41 and 47μM. On the other hand, titanium containing polyoxometalates (PMs-518, -520, -523) were not toxic at 100μM. The mechanism of anti-HIV action of PM-1001 was analyzed: it affected the binding of HIV to the cell membrane and syncytium formation between HIV-infected and uninfected cells.
AB - Seven polyoxotungstates substituted with vanadium or titanium atoms were examined for their activity against Flaviviridae (Dengue fever virus, DFV), Orthomyxoviridae (influenza virus type A, fluV-A), Paramyxoviridae (respiratory syncytial virus, RSV, parainfluenza virus type 2, PfluV-2 and canine distemper virus, CDV) and Lentiviridae (human immunodeficiency virus type 1, HIV-1) families. Among the seven polyoxotungstates examined, PM-43 {K5[SiVW11O40]}, PM-47 {K7[BVW11O40]}, and PM-1001 [K10Na(VO)3(SbW9O33) 2]26H2O contained vanadium. PM-1002 had the same core structure of (VO)3(SbW9O33)2 as PM-1001; however, three V atoms of PM-1001 consisted of two VIV and one VV and those of PM-1002 consisted of three VIV. On the other hand, PM-518 {[Et2NH2]7[PTi2W 10O40]}, PM-520 [Pri2NH2]5[PTiW11O40] and PM-523 [PriNH3]6H[PTi2W10O 38(O2)2]H2O all contained titanium. All compounds showed broad spectrum antiviral activity against all viruses examined except for PMs-43, -518 and -523 which did not exhibit inhibitory activity at ≥50μM against PfluV-2, CDV and DFV, respectively. All compounds were inhibitory against HIV replication at an EC50 of less than 2.0μM. Among them, PMs-1001 and -1002 showed the most potent inhibition. The compounds were not toxic for MDCK, HEp-2 and Vero cells at a concentration of 200μM. For the exponentially growing MT-4 cells, the vanadium containing polyoxometalates (PMs-43, 47, 1001, 1002) showed toxicity at concentrations between 41 and 47μM. On the other hand, titanium containing polyoxometalates (PMs-518, -520, -523) were not toxic at 100μM. The mechanism of anti-HIV action of PM-1001 was analyzed: it affected the binding of HIV to the cell membrane and syncytium formation between HIV-infected and uninfected cells.
KW - Antiviral
KW - Dengue fever virus
KW - HIV
KW - Polyoxometalate
KW - RNA virus
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U2 - 10.1016/S0166-3542(03)00009-3
DO - 10.1016/S0166-3542(03)00009-3
M3 - Article
C2 - 12767474
AN - SCOPUS:0038735360
VL - 58
SP - 265
EP - 271
JO - Antiviral Research
JF - Antiviral Research
SN - 0166-3542
IS - 3
ER -