Broad distribution and phylogeny of anaerobic endophytes of cluster XlVa clostridia in plant species including crops

Asami Saito, Makoto Kawahara, Seishi Ikeda, Masato Ishimine, Shoichiro Akao, Kiwam Minamisawa

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Endophytic Clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (TRFLP) analysis specific to the clostridial 16S rRNA gene. Endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. Phylogenetically, cluster XIVa clostridia were detected more frequently than cluster I clostridia in aerial parts. Isolation of clostridia from surface-sterilized sugarcane stem validated the TRFLP results. Plant-derived clostridia occupied two unique phylogenetic positions (groups I and II) within cluster XIVa. Most of cluster XIVa clostridia from other sources (e.g., human, animal, and insect intestines) were located outside these groups. Thus two unique groups of cluster XIVa clostridia are widely distributed in plants, including crops. In field-grown soybeans, TRFLP analysis revealed clostridia only in a non-nodulating mutant. Ribosomal intergenic spacer analysis (RISA) showed that the bacterial community in soybean shoot depended partly on the soybean nodulation genotype.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalMicrobes and environments
Volume23
Issue number1
DOIs
Publication statusPublished - 2008

Keywords

  • Clostridium
  • Cluster XIVa
  • Endophyte
  • Soybean
  • Sugarcane

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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