Breakdown of cytoskeletal proteins during meiosis of starfish oocytes and proteolysis induced by calpain

Luigia Santella, Keiichiro Kyozuka, Sjouke Hoving, Martin Munchbach, Manfredo Quadroni, Paola Dainese, Carlotta Zamparelli, Peter James, Ernesto Carafoli

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Meiosis reinitiation in starfish oocytes is characterized by Ca2+ transients in the cytosol and in the nucleus and is accompanied by the disassembly of the nuclear envelope, a process which is likely to be mediated by the cleavage of selected proteins. We have used mass spectrometry analysis (mass profile fingerprinting) on 2D polyacrylamide gels of extracts of oocytes in which meiosis resumption was induced by 1-methyladenine and have identified five proteins that were specifically degraded: α-tubulin, lamin B, dynamin, and two kinds of actin. They are all components of the cytoskeleton or associated with it. We then investigated whether calpain, which is activated by the increase in cell Ca2+, could cleave the same proteins that became degraded under the influence of 1-methyladenine and thus be involved in nuclear membrane breakdown. The investigation was prompted by the finding that microinjection of calpain into the nuclei of prophase arrested oocytes induced meiosis in the absence of 1-methyladenine. Incubation of prophase arrested (disrupted) oocytes with calpain produced a 2D gel protein pattern in which some of the degradation products coincided with those seen in oocytes challenged with 1-methyladenine. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalExperimental Cell Research
Volume259
Issue number1
DOIs
Publication statusPublished - 2000 Aug 25

Keywords

  • Calcium
  • Calpain
  • Nuclear envelope
  • Oocyte maturation
  • Proteolysis

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Breakdown of cytoskeletal proteins during meiosis of starfish oocytes and proteolysis induced by calpain'. Together they form a unique fingerprint.

Cite this