Abstract
A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.
Original language | English |
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Pages (from-to) | 131-135 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 400 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1997 Jan 2 |
Keywords
- active transport
- alanine, β-
- antiluminal uptake
- blood-brain barrier
- brain capillary endothelial cell
- taurine
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology