Brain-to-blood active transport of β-alanine across the blood-brain barrier

Junko Komura; Ikumi Tamai; Mizuho Senmaru; Tetsuya Terasaki; Yoshimichi Sai; Akira Tsuji

doi:10.1016/S0014-5793(96)01366-X

Brain-to-blood active transport of β-alanine across the blood-brain barrier

Junko Komura, Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Yoshimichi Sai, Akira Tsuji

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

Original language	English
Pages (from-to)	131-135
Number of pages	5
Journal	FEBS Letters
Volume	400
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(96)01366-X
Publication status	Published - 1997 Jan 2

Keywords

active transport
alanine, β-
antiluminal uptake
blood-brain barrier
brain capillary endothelial cell
taurine

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Brain-to-blood active transport of β-alanine across the blood-brain barrier",

abstract = "A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.",

keywords = "active transport, alanine, β-, antiluminal uptake, blood-brain barrier, brain capillary endothelial cell, taurine",

author = "Junko Komura and Ikumi Tamai and Mizuho Senmaru and Tetsuya Terasaki and Yoshimichi Sai and Akira Tsuji",

note = "Funding Information: The authors thank Mr. Khoichiro Kirihara, Meat Inspection Center of Kanazawa City (Kanazawa, Japan) for providing us with fresh bovine brains. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, grants from the Japan Health Sciences Foundation, Drug Innovation Project, and from Taisho Pharmaceutical Co., Ltd. ",

year = "1997",

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AU - Komura, Junko

AU - Tamai, Ikumi

AU - Senmaru, Mizuho

AU - Terasaki, Tetsuya

AU - Sai, Yoshimichi

AU - Tsuji, Akira

N1 - Funding Information: The authors thank Mr. Khoichiro Kirihara, Meat Inspection Center of Kanazawa City (Kanazawa, Japan) for providing us with fresh bovine brains. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, grants from the Japan Health Sciences Foundation, Drug Innovation Project, and from Taisho Pharmaceutical Co., Ltd.

PY - 1997/1/2

Y1 - 1997/1/2

N2 - A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

AB - A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

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KW - alanine, β-

KW - antiluminal uptake

KW - blood-brain barrier

KW - brain capillary endothelial cell

KW - taurine

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