Brain-to-blood active transport of β-alanine across the blood-brain barrier

Junko Komura; Ikumi Tamai; Mizuho Senmaru; Tetsuya Terasaki; Yoshimichi Sai; Akira Tsuji

doi:10.1016/S0014-5793(96)01366-X

Brain-to-blood active transport of β-alanine across the blood-brain barrier

Junko Komura, Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Yoshimichi Sai, Akira Tsuji

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

Original language	English
Pages (from-to)	131-135
Number of pages	5
Journal	FEBS Letters
Volume	400
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(96)01366-X
Publication status	Published - 1997 Jan 2

Keywords

active transport
alanine, β-
antiluminal uptake
blood-brain barrier
brain capillary endothelial cell
taurine

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(96)01366-X

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title = "Brain-to-blood active transport of β-alanine across the blood-brain barrier",

abstract = "A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.",

keywords = "active transport, alanine, β-, antiluminal uptake, blood-brain barrier, brain capillary endothelial cell, taurine",

author = "Junko Komura and Ikumi Tamai and Mizuho Senmaru and Tetsuya Terasaki and Yoshimichi Sai and Akira Tsuji",

note = "Funding Information: The authors thank Mr. Khoichiro Kirihara, Meat Inspection Center of Kanazawa City (Kanazawa, Japan) for providing us with fresh bovine brains. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, grants from the Japan Health Sciences Foundation, Drug Innovation Project, and from Taisho Pharmaceutical Co., Ltd.",

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doi = "10.1016/S0014-5793(96)01366-X",

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AU - Komura, Junko

AU - Tamai, Ikumi

AU - Senmaru, Mizuho

AU - Terasaki, Tetsuya

AU - Sai, Yoshimichi

AU - Tsuji, Akira

N1 - Funding Information: The authors thank Mr. Khoichiro Kirihara, Meat Inspection Center of Kanazawa City (Kanazawa, Japan) for providing us with fresh bovine brains. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, grants from the Japan Health Sciences Foundation, Drug Innovation Project, and from Taisho Pharmaceutical Co., Ltd.

PY - 1997/1/2

Y1 - 1997/1/2

N2 - A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

AB - A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

KW - active transport

KW - alanine, β-

KW - antiluminal uptake

KW - blood-brain barrier

KW - brain capillary endothelial cell

KW - taurine

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Brain-to-blood active transport of β-alanine across the blood-brain barrier

Abstract

Keywords

ASJC Scopus subject areas

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