Brain-to-blood active transport of β-alanine across the blood-brain barrier

Junko Komura, Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Yoshimichi Sai, Akira Tsuji

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A high-affinity antiluminal uptake system for β-alanine was demonstrated in primary cultured bovine brain capillary endothelial cells (BCEC) for which K(t) is 66.9 μM. β-alanine uptake was energy-, sodium- and chloride ion-dependent. β-amino acids strongly inhibited the uptake, while α- and γ-amino acids had a little or no inhibitory effect. In ATP-depleted cells, the uptake was stimulated by preloading β-alanine or taurine but not by L-leucine. These results suggest that β-alanine is actively transported across the antiluminal membrane of BCECs that is common to β-amino acids. The system may function for the efflux from the brain to blood.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalFEBS Letters
Volume400
Issue number1
DOIs
Publication statusPublished - 1997 Jan 2

Keywords

  • active transport
  • alanine, β-
  • antiluminal uptake
  • blood-brain barrier
  • brain capillary endothelial cell
  • taurine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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