Brain-specific receptor-type protein-tyrosine phosphatase RPTPβ is a chondroitin sulfate proteoglycan in vivo

Kenya Shitara, Hidekazu Yamada, Ken Watanabe, Motoyuki Shimonaka, Yu Yamaguchi

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    By using polyclonal antiserum, which recognizes multiple proteoglycan core proteins, we isolated a cDNA species for an unknown chondroitin sulfate proteoglycan in bovine brain. Unexpectedly, DNA sequencing revealed that the cDNA encodes an open reading frame highly homologous to the human receptor- type protein-tyrosine phosphatase, RPTPβ. To prove that RPTPβ is a proteoglycan, we raised three polyclonal antibodies against extracellular and cytoplasmic domains of human RPTPβ. These antibodies have been shown to react with a smear band ranging from 350 to 500 kDa in human brain extracts. Digestion with chondroitinase ABC eliminated this smear and gave rise to a 310/300-kDa doublet band that was not detected without digestion, indicating that almost all of the RPTPβ molecules in the brain contain chondroitin sulfate chains. In the cerebellum, immunofluorescence staining of chondroitinase-treated sections revealed pericellular localization of RPTPβ in the external and internal granular layers. These data establish that RPTPβ is expressed constitutively as a chondroitin sulfate proteoglycan in the brain, and suggest that chondroitin sulfates may be an essential component for the physiological function of RPTPβ in vivo.

    Original languageEnglish
    Pages (from-to)20189-20193
    Number of pages5
    JournalJournal of Biological Chemistry
    Volume269
    Issue number31
    Publication statusPublished - 1994 Aug 5

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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  • Cite this

    Shitara, K., Yamada, H., Watanabe, K., Shimonaka, M., & Yamaguchi, Y. (1994). Brain-specific receptor-type protein-tyrosine phosphatase RPTPβ is a chondroitin sulfate proteoglycan in vivo. Journal of Biological Chemistry, 269(31), 20189-20193.