Bradyrhizobium elkanii induces outer cortical root swelling in soybean

Ken Ichi Yuhashi; Shoichiro Akao; Hideyuki Fukuhara; Eiji Tateno; Jong Yoon Chun; Gary Stacey; Hiromichi Hara; Masatsugu Kubota; Teruo Asami; Kiwamu Minamisawa

Bradyrhizobium elkanii induces outer cortical root swelling in soybean

Ken Ichi Yuhashi, Shoichiro Akao, Hideyuki Fukuhara, Eiji Tateno, Jong Yoon Chun, Gary Stacey, Hiromichi Hara, Masatsugu Kubota, Teruo Asami, Kiwamu Minamisawa

LIF - Ecological Developmental Adaptability Life Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

When soybean roots were inoculated with Bradyrhizobium elkanii USDA76, USDA94 or USDA31, a localized swelling of the root outer cortical cells was observed within 10 days after inoculation. This phenotype was designated outer cortical swelling (OCS). OCS was not observed in roots inoculated with B.japonicum USDA110 or USDA122. Therefore, the OCS response appears to be specific for B. elkanii. To determine the involvement of the nodulation genes in OCS formation, we constructed a nod^- mutant, strain USDA94δNOD, of B. elkanii USDA94 in which the nodD₂D₁KABC genes were deleted. An OCS response was observed on roots inoculated with USDA94δNOD, indicating that the lipo-chitin nodulation signals synthesized by the nodABC gene products are not required for OCS formation.

Original language	English
Pages (from-to)	1571-1577
Number of pages	7
Journal	Plant and Cell Physiology
Volume	36
Issue number	8
Publication status	Published - 1995 Dec

Keywords

Bradyrhizobium elkanii
Nod factor
Root swelling
Soybean

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Cite this

Bradyrhizobium elkanii induces outer cortical root swelling in soybean. / Yuhashi, Ken Ichi; Akao, Shoichiro; Fukuhara, Hideyuki; Tateno, Eiji; Chun, Jong Yoon; Stacey, Gary; Hara, Hiromichi; Kubota, Masatsugu; Asami, Teruo; Minamisawa, Kiwamu.

In: Plant and Cell Physiology, Vol. 36, No. 8, 12.1995, p. 1571-1577.

Research output: Contribution to journal › Article › peer-review

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title = "Bradyrhizobium elkanii induces outer cortical root swelling in soybean",

abstract = "When soybean roots were inoculated with Bradyrhizobium elkanii USDA76, USDA94 or USDA31, a localized swelling of the root outer cortical cells was observed within 10 days after inoculation. This phenotype was designated outer cortical swelling (OCS). OCS was not observed in roots inoculated with B.japonicum USDA110 or USDA122. Therefore, the OCS response appears to be specific for B. elkanii. To determine the involvement of the nodulation genes in OCS formation, we constructed a nod- mutant, strain USDA94δNOD, of B. elkanii USDA94 in which the nodD2D1KABC genes were deleted. An OCS response was observed on roots inoculated with USDA94δNOD, indicating that the lipo-chitin nodulation signals synthesized by the nodABC gene products are not required for OCS formation.",

keywords = "Bradyrhizobium elkanii, Nod factor, Root swelling, Soybean",

author = "Yuhashi, {Ken Ichi} and Shoichiro Akao and Hideyuki Fukuhara and Eiji Tateno and Chun, {Jong Yoon} and Gary Stacey and Hiromichi Hara and Masatsugu Kubota and Teruo Asami and Kiwamu Minamisawa",

note = "Funding Information: This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan (no. 06660073). Work in the USA was supported by grant 92ER20072 from the U.S. Department of Energy (to OS). We are grateful to DT. E.W. Trip-plet (Wisconsin University) for the physical maps of the USDA94 nod gene region, to Mr. Y. Minakawa (Ibaraki University) for the preparation of sections for light microscopy, and to Dr. T. Mat-suda (Ibaraki University) for his valuable suggestions.",

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AU - Yuhashi, Ken Ichi

AU - Akao, Shoichiro

AU - Fukuhara, Hideyuki

AU - Tateno, Eiji

AU - Chun, Jong Yoon

AU - Stacey, Gary

AU - Hara, Hiromichi

AU - Kubota, Masatsugu

AU - Asami, Teruo

AU - Minamisawa, Kiwamu

N1 - Funding Information: This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan (no. 06660073). Work in the USA was supported by grant 92ER20072 from the U.S. Department of Energy (to OS). We are grateful to DT. E.W. Trip-plet (Wisconsin University) for the physical maps of the USDA94 nod gene region, to Mr. Y. Minakawa (Ibaraki University) for the preparation of sections for light microscopy, and to Dr. T. Mat-suda (Ibaraki University) for his valuable suggestions.

PY - 1995/12

Y1 - 1995/12

N2 - When soybean roots were inoculated with Bradyrhizobium elkanii USDA76, USDA94 or USDA31, a localized swelling of the root outer cortical cells was observed within 10 days after inoculation. This phenotype was designated outer cortical swelling (OCS). OCS was not observed in roots inoculated with B.japonicum USDA110 or USDA122. Therefore, the OCS response appears to be specific for B. elkanii. To determine the involvement of the nodulation genes in OCS formation, we constructed a nod- mutant, strain USDA94δNOD, of B. elkanii USDA94 in which the nodD2D1KABC genes were deleted. An OCS response was observed on roots inoculated with USDA94δNOD, indicating that the lipo-chitin nodulation signals synthesized by the nodABC gene products are not required for OCS formation.

AB - When soybean roots were inoculated with Bradyrhizobium elkanii USDA76, USDA94 or USDA31, a localized swelling of the root outer cortical cells was observed within 10 days after inoculation. This phenotype was designated outer cortical swelling (OCS). OCS was not observed in roots inoculated with B.japonicum USDA110 or USDA122. Therefore, the OCS response appears to be specific for B. elkanii. To determine the involvement of the nodulation genes in OCS formation, we constructed a nod- mutant, strain USDA94δNOD, of B. elkanii USDA94 in which the nodD2D1KABC genes were deleted. An OCS response was observed on roots inoculated with USDA94δNOD, indicating that the lipo-chitin nodulation signals synthesized by the nodABC gene products are not required for OCS formation.

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Bradyrhizobium elkanii induces outer cortical root swelling in soybean

Abstract

Keywords

ASJC Scopus subject areas

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