Bradykinin regulation of airway submucosal gland secretion: Role of bradykinin receptor subtype

M. Nagaki, S. Shimura, T. Irokawa, T. Sasaki, T. Oshiro, M. Nara, Y. Kakuta, K. Shirato

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20 Citations (Scopus)

Abstract

To clarify the role of bradykinin receptor subtypes, we examined the effect of bradykinin on feline tracheal and human airway submucosal gland secretion using an isolated gland preparation. Bradykinin induced a significant increase in [3H]glycoconjugate secretion in a dose-dependent manner from isolated glands, which was significantly inhibited by D-Arg- (Hyp3,Thi58,D-Phe7)-bradykinin (the B2-receptor antagonist), whereas Des-Arg9(Leu8)-bradykinin (B1-receptor antagonist) or indomethacin did not significantly alter it. Nitric oxide synthase inhibitor (nitro-L-arginine methyl ester) caused a significant inhibition of bradykinin-induced glycoconjugate secretion, which was reversed by the addition of L-arginine. Bradykinin evoked bidirectional current responses, and an initial inward current (Cl- current) was followed by an outward current (K+ current) of the acinar cells in a whole cell configuration by patch-clamp technique. Bradykinin induced an immediate increase in intracellular calcium concentration ([Ca2+](i)) of the acinar cells followed by a prolonged plateau, and Ca2+ removal resulted in an initial increase alone. [Ca2+](i) rise was significantly inhibited by the B2-receptor antagonist, whereas the B1-receptor antagonist did not significantly alter it. These findings suggest that B-receptor stimulation and the resultant [Ca2+](i) rise induced both mucus glycoprotein and electrolyte secretions, involving NO formation in airway submucosal gland cells.

Original languageEnglish
Pages (from-to)L907-L913
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume270
Issue number6 14-6
DOIs
Publication statusPublished - 1996 Jun

Keywords

  • B-receptor
  • B-receptor
  • chloride
  • feline
  • human
  • intracellular calcium concentration
  • mucus glycoprotein secretion
  • secretion
  • submucosal gland cells

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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