Both bovine myeloperoxidase and lactoperoxidase contain one calcium per iron with no other metal present in significant amount. Calcium is bound with high affinity and is removed upon exposure to 6 M guanidine hydrochloride/EGTA which results in precipitation of protein. Computer amino acid sequence analyses of human myeloperoxidase reveal two plausible calcium binding sites. This is the first evidence for the presence of calcium in these peroxidases.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1989 Apr 28|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology