Bovine myeloperoxidase and lactoperoxidase each contain a high affinity site for calcium

Karla S. Booth, Shioko Kimura, H. Caroline Lee, Masao Ikeda-Saito, Winslow S. Caughey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Both bovine myeloperoxidase and lactoperoxidase contain one calcium per iron with no other metal present in significant amount. Calcium is bound with high affinity and is removed upon exposure to 6 M guanidine hydrochloride/EGTA which results in precipitation of protein. Computer amino acid sequence analyses of human myeloperoxidase reveal two plausible calcium binding sites. This is the first evidence for the presence of calcium in these peroxidases.

Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalBiochemical and biophysical research communications
Volume160
Issue number2
DOIs
Publication statusPublished - 1989 Apr 28

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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