Bovine anterior pituitary progenitor cell line expresses interleukin (IL)-18 and IL-18 receptor

Y. Nagai, H. Ogasawara, Y. Taketa, Hisashi Aso, S. Tanaka, T. Kanaya, Kouichi Watanabe, S. Ohwada, Y. Muneta, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


In the anterior pituitary gland, inflammatory mediators regulate cell function through an immuno-endocrine pathway. Recent studies have shown that undifferentiated stem cells act as immunomodulators. These studies prompted us to establish a progenitor cell line from the bovine anterior pituitary gland and to detail its function. First, we localised interleukin (IL)-18 by immunohistochemistry to the marginal cell layer of Rathke's pouch that is assumed to embody a stem/progenitor cell compartment of the postnatal pituitary gland. A cloned anterior pituitary-derived cell line from the bovine anterior pituitary gland was established from single cell clone by the limiting dilution method and was designated as bovine anterior pituitary-derived cell line (BAPC)-1. BAPC-1 cells constantly expressed mRNAs for IL-18 and IL-18 receptor, and grew steadily and rapidly in the medium containing epidermal growth factor and basic fibroblast growth factor. The cell line also expressed the mRNAs for the stem/progenitor cell- related factors such as Nanog, Oct-4, Ptch1, Nestin, Notch1, Hes1, Lrp and Fzd4, and the mRNAs for embryonic pituitary-related factors, such as Lhx3, PitX1 and Pit-1. The nuclei of BAPC-1 were immunostained positively for Pit-1, Hes1 and β-catenin antibodies. Furthermore, BAPC-1 cells expressed mRNAs for cytokine such as IL-1α, IL-6, IL-7, IL-12 and IL-15. Stimulation of BAPC-1 cells with IL-18 increased expression of mRNAs for IL-1α, IL-6, IL-1β and IL-8. At day 6 in culture, BAPC-1 cells also express growth hormone mRNA. These results strongly suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its cytokine production.

Original languageEnglish
Pages (from-to)1233-1241
Number of pages9
JournalJournal of Neuroendocrinology
Issue number11
Publication statusPublished - 2008 Oct 21


  • Anterior pituitary
  • Cell line
  • IL-18
  • Immune-endocrine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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