TY - JOUR
T1 - Borrelia burgdorferi sensu lato in an endemic environment
T2 - Wild sika deer (Cervus nippon yesoensis) with infected ticks and antibodies
AU - Isogai, Emiko
AU - Isogai, Hiroshi
AU - Masuzawa, Toshiyuki
AU - Postic, Daniele
AU - Baranton, Guy
AU - Kamewaka, Yuri
AU - Kimura, Koichi
AU - Nishikawa, Takeshi
AU - Fujii, Nobuhiro
AU - Ishii, Norihisa
AU - Ohno, Shigeaki
AU - Yamaguti, Noboru
PY - 1996
Y1 - 1996
N2 - Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I, ovatus (adult) were the common ticks on sika deer, Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature.
AB - Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I, ovatus (adult) were the common ticks on sika deer, Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature.
KW - Borrelia burgdorferi sensu lato
KW - Ixodes ticks
KW - Lyme disease
KW - Sika deer
UR - http://www.scopus.com/inward/record.url?scp=9044234016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9044234016&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.1996.tb03311.x
DO - 10.1111/j.1348-0421.1996.tb03311.x
M3 - Article
C2 - 8871523
AN - SCOPUS:9044234016
VL - 40
SP - 13
EP - 19
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 1
ER -