Bootstrap resampling technique to evaluate the reliability of the optimal liposome formulation: Skin permeability and stability response variables

Sureewan Duangjit, Praneet Opanasopit, Theerasak Rojanarata, Jun Takayama, Kozo Takayama, Tanasait Ngawhirunpat

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A nonlinear response surface method incorporating multivariate spline interpolation (RSM-S) is a useful technique for the optimization of pharmaceutical formulations, although the direct reliability of the optimal formulation must be evaluated. In this study, we demonstrated the feasibility of using the bootstrap (BS) resampling technique to evaluate the direct reliability of the optimal liposome formulation predicted by RSM-S. The formulation characteristics (Xn), including vesicle size (X1), size distribution (X2), zeta potential (X3), elasticity (X4), drug content (X5), entrapment efficiency (X6), release rate (X7), and the penetration enhancer (PE) factors as formulation factors (Zn), with the type of PE (Z1) and content of PE (Z2) were used as causal factors of the response surface analysis. The intended responses were high skin permeability (flux, Y1) and high stability formulation (drug remaining, Y2). Based on the dataset obtained, the simultaneous optimal solutions were estimated using RSM-S. Leave-one-out-cross-validation showed satisfying reliability of the optimal solution. Concurrently, similar BS optimal solutions were estimated from the BS dataset that was generated from the original dataset through BS resampling at frequencies of 250, 500, 750, and 1000. The analysis and simulation indicated that X4, X5, and Z2were the prime factors affecting Y1and Y2. These findings suggest that this approach could also be useful for evaluating the reliability of an optimal liposome formulation predicted by RSM-S and would be beneficial for the pharmaceutical development of liposomes for transdermal drug delivery.

Original languageEnglish
Pages (from-to)1543-1549
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number9
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

Keywords

  • Bootstrap
  • Response surface
  • Simultaneous optimal solution
  • Transdermal drug delivery

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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