Bone marrow stromal cells selectively stimulate the rapid expansion of lineage‐restricted myeloid progenitors

Jun‐Ichi ‐I Kameoka, Nobuaki Yanai, Masuo Obinata

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42 Citations (Scopus)

Abstract

Bone marrow stromal cells serve hematopoietic microenvironments where different blood cells are controlled in their growth and differentiation. To characterize functions of stromal cells, 33 bone marrow stromal cells including preadipocytes, endothelial cells, and fibroblasts were established from transgenic mice harboring temperature‐sensitive SV40 T‐antigen gene and their selective stimulatory abilities to support large colony formation of lineage‐specific hematopoietic progenitor cells (erythroid, monocyte/macrophage, granulocyte, and monocyte‐granulocyte) were examined. Among established stromal cells, 27 clones showed erythropoietic stimulatory activity in the presence of erythropoietin. On myeloid progenitors, the stromal cells showed lineage‐restricted stimulatory activity and a reciprocal relationship was observed between granulocyte formation and macrophage formation, but these activities were not dependent on the amount of produced colony‐stimulating factors (CSFs). Our present study with many stromal cells established from bone marrow indicated that each stromal cell in the bone marrow may provide the preferable microenvironment for a rapid expansion of the lineage‐restricted progenitor cells in combination with CSFs. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalJournal of Cellular Physiology
Volume164
Issue number1
DOIs
Publication statusPublished - 1995 Jul

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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