Bone marrow oxytocin mediates the anabolic action of estrogen on the skeleton

Graziana Colaianni, Li Sun, Adriana Di Benedetto, Roberto Tamma, Ling Ling Zhu, Jay Cao, Maria Grano, Tony Yuen, Sylvia Colucci, Concetta Cuscito, Lucia Mancini, Jianhua Li, Katsuhiko Nishimori, Itai Bab, Heon Jin Lee, Jameel Iqbal, W. Scott Young, Clifford Rosen, Alberta Zallone, Mone Zaidi

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Estrogen uses two mechanisms to exert its effect on the skeleton: it inhibits bone resorption by osteoclasts and, at higher doses, can stimulate bone formation. Although the antiresorptive action of estrogen arises from the inhibition of the MAPK JNK, the mechanism of its effect on the osteoblast remains unclear. Here, we report that the anabolic action of estrogen in mice occurs, at least in part, through oxytocin (OT) produced by osteoblasts in bone marrow. We show that the absence of OT receptors (OTRs) in OTR-/- osteoblasts or attenuation of OTR expression in silenced cells inhibits estrogen-induced osteoblast differentiation, transcription factor up-regulation, and/or OT production in vitro. In vivo, OTR-/- mice, known to have a bone formation defect, fail to display increases in trabecular bone volume, cortical thickness, and bone formation in response to estrogen. Furthermore, osteoblast-specific Col2.3-Cre+/OTRfl/fl mice, but not TRAP-Cre+/OTRfl/fl mice, mimic the OTR-/- phenotype and also fail to respond to estrogen. These data attribute the phenotype of OTR deficiency to an osteoblastic rather than an osteoclastic defect. Physiologically, feed-forward OT release in bone marrow by a rising estrogen concentration may facilitate rapid skeletal recovery during the latter phases of lactation.

Original languageEnglish
Pages (from-to)29159-29167
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
Publication statusPublished - 2012 Aug 17

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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