BMP-2 release and dose-response studies in hydroxyapatite and β-tricalcium phosphate

Junichi Tazaki, Masaru Murata, Toshiyuki Akazawa, Masaya Yamamoto, Katsutoshi Ito, Makoto Arisue, Takanori Shibata, Yasuhiko Tabata

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

The purpose of this study is to compare in vivo retention of BMP-2 and bone induction in HAp (porosity: 60-80%, pore size: 100-600 μm, sintering temperature: 800°C, surface area: 1 m2/g) and β-TCP (porosity: 75%, pore size: 100-400 μm, sintering temperature: 1050°C, surface area: 4 m2/g). We estimated the in vivo release profile of 125I-labeled BMP-2 and bone induction of hard tissues histologically. The amount of BMP-2 remaining in the β-TCP at 1 day after implantation was 49.6%, while the amount was 34.0% in the HAp. Furthermore, the HAp and β-TCP containing 0.0, 0.05, 0.1, 0.3, 0.5, 1.0, 5.0 μg of BMP-2 were implanted into the back subcutis of 4-week old Wistar rats. At 3 weeks after implantation, the ceramics were explanted and evaluated histologically. The HAp/BMP-2 (5.0 μg) system showed 3.0% in the total volume of bone at 3 weeks, while only in the β-TCP/BMP-2 (5.0 μg) system showed 32.5%. These results indicate that the absorbable β-TCP block may be an effective bioceramic for bone induction to deliver BMP-2 to the site of action.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalBio-medical materials and engineering
Volume19
Issue number2-3
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • BMP-2
  • Dose-response
  • Hydroxyapatite
  • Release
  • β-tricalcium phosphate

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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