BMP-2 dose-response and release studies in functionally graded HAp

Junichi Tazaki, Toshiyuki Akazawa, Masaru Murata, Masaya Yamamoto, Yasuhiko Tabata, Ryota Yoshimoto, Makoto Arisue

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Hydroxyapatite (HAp) has been used as a biomaterial for hard tissues. Critical characteristics of biomaterials will include surface geometry, hydrophobicity and hydrophilicity, crystallinity, biodegradation rates, and release pharmacokinetics (PK) of incorporated molecules such as BMP-2. Optimizing BMP-2 for clinical application may be dependent on localized sustained release from biomaterials. We forcused on in vivo local BMP-2 PK and bone induction in two ceramics systems, based on different surface structures. The functionally graded apatites (fg-HAp) was designed by the step-wise calcinations and partial dissolution-precipitation methods. We estimated the in vivo release profile of 125I-labeled BMP-2 from fg-HAp and the dose response of bone induction by BMP-2 in the back subcutis histologically. Bulk-HAp (b-HAp) by only the step-wise calcinations was prepared as a control. The amount of BMP-2 remaining in the fg-HAp at 1 day after implantation was 83.8%, while that was 34.6% in the b-HAp. Moreover, ectopic bone formation were found surely in the fg-HAp/BMP-2 (0.5 μ g) system at 3 weeks, not in the b-HAp/BMP-2 system. By using fg-HAp, it is likely that an extremely low dose of BMP-2 is enough to enhance bone induction if BMP-2 is appropriately delivered to the site of action.

Original languageEnglish
Pages (from-to)965-968
Number of pages4
JournalKey Engineering Materials
Volume309-311 II
Publication statusPublished - 2006
Externally publishedYes


  • BMP-2
  • Dose-response
  • Functionally graded HAp
  • Release

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering


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