BmEts upregulates promoter activity of lebocin in Bombyx mori

Hiromitsu Tanaka; Aki Sagisaka; Kosuke Fujita; Seiichi Furukawa; Jun Ishibashi; Minoru Yamakawa

doi:10.1016/j.ibmb.2012.03.004

BmEts upregulates promoter activity of lebocin in Bombyx mori

Hiromitsu Tanaka, Aki Sagisaka, Kosuke Fujita, Seiichi Furukawa, Jun Ishibashi, Minoru Yamakawa

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The Ets family protein BmEts is assumed to be implicated in determination of diapause in the embryogenesis of . Bombyx mori. In this study, we found that expression of . BmEts was increased in the fat body and other tissues of the 5th instar larvae in response to . Escherichia coli injection. Cotransfection experiments using a silkworm cell line revealed that overexpression of BmEts significantly elevated the activity of . lebocin promoter but not of . cecropin B1, . cecropin D, . attacin, and . moricin promoters. Activation of the . lebocin promoter by BmEts was dependent on at least two κB elements and the most proximal GGAA/T motif located on the 5'-upstream region. BmEts further synergistically enhanced . E. coli or BmRelish1-d2 (active form)-stimulated . lebocin promoter activation. Two κB elements were also found to be involved in promoter activation by BmRelish1-d2 and in synergistic promoter activation by BmEts and BmRelish1-d2 in the silkworm cells. Specific binding of recombinant BmEts to the proximal κB element and the most proximal GGAA/T motif and interaction between BmEts and BmRelish1 were also observed. To our knowledge, this is the first report of an Ets family protein directly regulating immune-related genes in invertebrates.

Original language	English
Pages (from-to)	474-481
Number of pages	8
Journal	Insect Biochemistry and Molecular Biology
Volume	42
Issue number	7
DOIs	https://doi.org/10.1016/j.ibmb.2012.03.004
Publication status	Published - 2012 Jul
Externally published	Yes

Keywords

Antimicrobial peptides
Bombyx mori
Ets family protein
Gene regulation
Silkworm

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

Access to Document

10.1016/j.ibmb.2012.03.004

Cite this

@article{2e12c7493bbb47ecb100195d899b3552,

title = "BmEts upregulates promoter activity of lebocin in Bombyx mori",

abstract = "The Ets family protein BmEts is assumed to be implicated in determination of diapause in the embryogenesis of . Bombyx mori. In this study, we found that expression of . BmEts was increased in the fat body and other tissues of the 5th instar larvae in response to . Escherichia coli injection. Cotransfection experiments using a silkworm cell line revealed that overexpression of BmEts significantly elevated the activity of . lebocin promoter but not of . cecropin B1, . cecropin D, . attacin, and . moricin promoters. Activation of the . lebocin promoter by BmEts was dependent on at least two κB elements and the most proximal GGAA/T motif located on the 5'-upstream region. BmEts further synergistically enhanced . E. coli or BmRelish1-d2 (active form)-stimulated . lebocin promoter activation. Two κB elements were also found to be involved in promoter activation by BmRelish1-d2 and in synergistic promoter activation by BmEts and BmRelish1-d2 in the silkworm cells. Specific binding of recombinant BmEts to the proximal κB element and the most proximal GGAA/T motif and interaction between BmEts and BmRelish1 were also observed. To our knowledge, this is the first report of an Ets family protein directly regulating immune-related genes in invertebrates.",

keywords = "Antimicrobial peptides, Bombyx mori, Ets family protein, Gene regulation, Silkworm",

author = "Hiromitsu Tanaka and Aki Sagisaka and Kosuke Fujita and Seiichi Furukawa and Jun Ishibashi and Minoru Yamakawa",

note = "Funding Information: We are grateful to Gene Bank of the National Institute of Agrobiological Sciences for providing a silkworm cell line. We thank Dr. Kazuei Mita (National Institute of Agrobiological Sciences) and Prof. Toru Shimada (University of Tokyo) for providing the BmEts cDNA clone. This work was supported in part by a Grant-in-Aid for Scientific Research (B) ( 21380043 to H.T.). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

month = jul,

doi = "10.1016/j.ibmb.2012.03.004",

language = "English",

volume = "42",

pages = "474--481",

journal = "Insect Biochemistry and Molecular Biology",

issn = "0965-1748",

publisher = "Elsevier Limited",

number = "7",

}

TY - JOUR

T1 - BmEts upregulates promoter activity of lebocin in Bombyx mori

AU - Tanaka, Hiromitsu

AU - Sagisaka, Aki

AU - Fujita, Kosuke

AU - Furukawa, Seiichi

AU - Ishibashi, Jun

AU - Yamakawa, Minoru

N1 - Funding Information: We are grateful to Gene Bank of the National Institute of Agrobiological Sciences for providing a silkworm cell line. We thank Dr. Kazuei Mita (National Institute of Agrobiological Sciences) and Prof. Toru Shimada (University of Tokyo) for providing the BmEts cDNA clone. This work was supported in part by a Grant-in-Aid for Scientific Research (B) ( 21380043 to H.T.). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/7

Y1 - 2012/7

N2 - The Ets family protein BmEts is assumed to be implicated in determination of diapause in the embryogenesis of . Bombyx mori. In this study, we found that expression of . BmEts was increased in the fat body and other tissues of the 5th instar larvae in response to . Escherichia coli injection. Cotransfection experiments using a silkworm cell line revealed that overexpression of BmEts significantly elevated the activity of . lebocin promoter but not of . cecropin B1, . cecropin D, . attacin, and . moricin promoters. Activation of the . lebocin promoter by BmEts was dependent on at least two κB elements and the most proximal GGAA/T motif located on the 5'-upstream region. BmEts further synergistically enhanced . E. coli or BmRelish1-d2 (active form)-stimulated . lebocin promoter activation. Two κB elements were also found to be involved in promoter activation by BmRelish1-d2 and in synergistic promoter activation by BmEts and BmRelish1-d2 in the silkworm cells. Specific binding of recombinant BmEts to the proximal κB element and the most proximal GGAA/T motif and interaction between BmEts and BmRelish1 were also observed. To our knowledge, this is the first report of an Ets family protein directly regulating immune-related genes in invertebrates.

AB - The Ets family protein BmEts is assumed to be implicated in determination of diapause in the embryogenesis of . Bombyx mori. In this study, we found that expression of . BmEts was increased in the fat body and other tissues of the 5th instar larvae in response to . Escherichia coli injection. Cotransfection experiments using a silkworm cell line revealed that overexpression of BmEts significantly elevated the activity of . lebocin promoter but not of . cecropin B1, . cecropin D, . attacin, and . moricin promoters. Activation of the . lebocin promoter by BmEts was dependent on at least two κB elements and the most proximal GGAA/T motif located on the 5'-upstream region. BmEts further synergistically enhanced . E. coli or BmRelish1-d2 (active form)-stimulated . lebocin promoter activation. Two κB elements were also found to be involved in promoter activation by BmRelish1-d2 and in synergistic promoter activation by BmEts and BmRelish1-d2 in the silkworm cells. Specific binding of recombinant BmEts to the proximal κB element and the most proximal GGAA/T motif and interaction between BmEts and BmRelish1 were also observed. To our knowledge, this is the first report of an Ets family protein directly regulating immune-related genes in invertebrates.

KW - Antimicrobial peptides

KW - Bombyx mori

KW - Ets family protein

KW - Gene regulation

KW - Silkworm

UR - http://www.scopus.com/inward/record.url?scp=84861454690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861454690&partnerID=8YFLogxK

U2 - 10.1016/j.ibmb.2012.03.004

DO - 10.1016/j.ibmb.2012.03.004

M3 - Article

C2 - 22484450

AN - SCOPUS:84861454690

VL - 42

SP - 474

EP - 481

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 7

ER -

BmEts upregulates promoter activity of lebocin in Bombyx mori

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this