Abstract
A functionally graded apatite (fg-HAp) with body fluid permeability was developed from bovine bone. The tissue reaction of fg-HAp and its efficacy as a scaffold for recombinant human bone morphogenetic protein-2 (BMP-2) were evaluated histomorphometrically, and a component of permeable fluid into the fg-HAp was analyzed by immunoblotting assay. The fg-HAp block (27 mm 3) combined with and without BMP-2 (5 μg) was implanted subcutaneously in 4-week-old Wistar rats. Histological examination showed that the surface and bulk degradations of the fg-HAp proceeded extensively and giant cells appeared on the fg-HAp at 2 weeks. Body fluid permeation was found inside the fg-HAp, and the fluid component was immunopositive for albumin. In addition, albumin was detected as a main component among proteins collected from the in vivo implanted fg-HAp. The bioabsorption of the fg-HAp was accelerated as BMP-2-induced bone matured. Histomorphometrical analysis at 4 weeks in the BMP-2/fg-HAp implant showed 59.0% in the total volume of bone and marrow. These results indicate that fg-HAp is an innovative, bioabsorbable bioceramic with fluid permeability characteristic, and may become a biointegrated scaffold for bone engineering.
Original language | English |
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Pages (from-to) | 469-475 |
Number of pages | 7 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 81 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2007 May |
Externally published | Yes |
Keywords
- BMP-2
- Bioabsorption albumin
- Body fluid
- Functionally graded apatite
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering