Blood permeability of a novel ceramic scaffold for bone morphogenetic protein-2

M. Murata, T. Akazawa, J. Tazaki, K. Ito, T. Sasaki, Masaya Yamamoto, Y. Tabata, M. Arisue

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A functionally graded apatite (fg-HAp) with body fluid permeability was developed from bovine bone. The tissue reaction of fg-HAp and its efficacy as a scaffold for recombinant human bone morphogenetic protein-2 (BMP-2) were evaluated histomorphometrically, and a component of permeable fluid into the fg-HAp was analyzed by immunoblotting assay. The fg-HAp block (27 mm 3) combined with and without BMP-2 (5 μg) was implanted subcutaneously in 4-week-old Wistar rats. Histological examination showed that the surface and bulk degradations of the fg-HAp proceeded extensively and giant cells appeared on the fg-HAp at 2 weeks. Body fluid permeation was found inside the fg-HAp, and the fluid component was immunopositive for albumin. In addition, albumin was detected as a main component among proteins collected from the in vivo implanted fg-HAp. The bioabsorption of the fg-HAp was accelerated as BMP-2-induced bone matured. Histomorphometrical analysis at 4 weeks in the BMP-2/fg-HAp implant showed 59.0% in the total volume of bone and marrow. These results indicate that fg-HAp is an innovative, bioabsorbable bioceramic with fluid permeability characteristic, and may become a biointegrated scaffold for bone engineering.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume81
Issue number2
DOIs
Publication statusPublished - 2007 May 1
Externally publishedYes

Keywords

  • BMP-2
  • Bioabsorption albumin
  • Body fluid
  • Functionally graded apatite

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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